PMID- 1807029 OWN - NLM STAT- MEDLINE DCOM- 19920513 LR - 20161026 IS - 0375-8427 (Print) IS - 0375-8427 (Linking) VI - 36 IP - 6 DP - 1991 Jun TI - [Lactic acid production and urease activity in strains of Enterococcus faecium found in the rumen and their genetic stability]. PG - 335-40 AB - Lactic acid production, urease activity and genetic stability were investigated in five selected rumen strains of Enterococcus faecium. The average value of urease activity in the tested strains was 16.5 +/- 0.953 nkat per ml, two strains were urease-negative. The values of E. faecium strains produced lactic acid ranged from 1.087 +/- 0.134 to 1.787 +/- 0.213 mmol per 1 l. Cultivation of the strains in ethidium bromide (EB) eliminated urease activity of these strains already in the first subculture, but the elimination effects of sodium dodecyl sulphate (SDS) and acridine orange (AO) were manifested later on (1-8 subcultures). Lactic acid production was eliminated in all strains from 1st to 8th subculture after cultivation in SDS, EB and also AO. FAU - Laukova, A AU - Laukova A AD - Ustav fyziologie hospodarskych zvierat SAV, Kosice. FAU - Kuncova, M AU - Kuncova M LA - slo PT - English Abstract PT - Journal Article TT - Produkcia kyseliny mliecnej a ureazova aktivita u bachorovych kmenov Enterococcus faecium a ich geneticka stabilita. PL - Czech Republic TA - Vet Med (Praha) JT - Veterinarni medicina JID - 0063417 RN - 0 (Lactates) RN - 33X04XA5AT (Lactic Acid) RN - EC 3.5.1.5 (Urease) SB - IM MH - Animals MH - Cattle/*microbiology MH - Enterococcus faecium/genetics/isolation & purification/*metabolism MH - Lactates/*biosynthesis MH - Lactic Acid MH - Rumen/*microbiology MH - Sheep/*microbiology MH - Urease/*metabolism EDAT- 1991/06/01 00:00 MHDA- 1991/06/01 00:01 CRDT- 1991/06/01 00:00 PHST- 1991/06/01 00:00 [pubmed] PHST- 1991/06/01 00:01 [medline] PHST- 1991/06/01 00:00 [entrez] PST - ppublish SO - Vet Med (Praha). 1991 Jun;36(6):335-40.