PMID- 18071843
OWN - NLM
STAT- MEDLINE
DCOM- 20080815
LR  - 20211203
IS  - 1438-2199 (Electronic)
IS  - 0939-4451 (Linking)
VI  - 34
IP  - 4
DP  - 2008 May
TI  - Hydrogen sulfide inhibits myocardial injury induced by homocysteine in rats.
PG  - 573-85
AB  - Hyperhomocysteinemia (HHcy) is a critical independent risk factor for 
      cardiovascular diseases. However, to date, no satisfactory strategies to prevent 
      HHcy exist. Since homocysteine (Hcy) and endogenous H2S are both metabolites of 
      sulfur-containing amino acids, we aimed to investigate whether a metabolic 
      product of Hcy and H2S, may antagonize in part the cardiovascular effects of Hcy. 
      In the HHcy rat model injected subcutaneously with Hcy for 3 weeks, H2S levels 
      and the H2S-generating enzyme cystathionine gamma lyase (CSE) activity in the 
      myocardium were decreased. The intraperitoneal injection of H2S gas saturation 
      solution significantly reduced plasma total Hcy (tHcy) concentration and 
      decreased lipid peroxidation formation (i.e., lowered manodialdehyde and 
      conjugated diene levels in myocardia and plasma). The activities of myocardial 
      mitochondrial respiratory enzymes succinate dehydrogenase, cytochrome oxidase, 
      and manganese superoxide dismutase, related to reactive oxygen species 
      metabolism, were significantly dysfunctional in HHcy rats. The H2S administration 
      restored the level of enzyme activities and accelerated the scavenging of H2O2 
      and superoxide anion generated by Hcy in isolated mitochondria. The H2S treatment 
      also inhibited the expression of glucose-regulated protein 78, a marker of 
      endoplasmic reticulum (ER) stress, induced by Hcy in vivo and in vitro. Thus, 
      HHcy impaired the myocardial CSE/H2S pathway, and the administration of H2S 
      protected the myocardium from oxidative and ER stress induced by HHcy, which 
      suggests that an endogenous metabolic balance of sulfur-containing amino acids 
      may be a novel strategy for treatment of HHcy.
FAU - Chang, Lin
AU  - Chang L
AD  - Institute of Cardiovascular Research, Peking University, Xishuku Street 8, West 
      District, 100034 Beijing, People's Republic of China.
FAU - Geng, Bin
AU  - Geng B
FAU - Yu, Fang
AU  - Yu F
FAU - Zhao, Jing
AU  - Zhao J
FAU - Jiang, Hongfeng
AU  - Jiang H
FAU - Du, Junbao
AU  - Du J
FAU - Tang, Chaoshu
AU  - Tang C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071211
PL  - Austria
TA  - Amino Acids
JT  - Amino acids
JID - 9200312
RN  - 0 (Endoplasmic Reticulum Chaperone BiP)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Molecular Chaperones)
RN  - 0 (RNA, Messenger)
RN  - 0 (Reactive Oxygen Species)
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - 11062-77-4 (Superoxides)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 1.3.99.1 (Succinate Dehydrogenase)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
RN  - EC 4.4.1.1 (Cystathionine gamma-Lyase)
RN  - YY9FVM7NSN (Hydrogen Sulfide)
SB  - IM
EIN - Amino Acids. 2008 May;34(4):687
MH  - Animals
MH  - Cystathionine gamma-Lyase/*drug effects/metabolism
MH  - Disease Models, Animal
MH  - Electron Transport Complex IV/drug effects/metabolism
MH  - Endoplasmic Reticulum/drug effects/metabolism
MH  - Endoplasmic Reticulum Chaperone BiP
MH  - Enzyme Activation/drug effects
MH  - Heart Diseases/*prevention & control
MH  - Heat-Shock Proteins/drug effects/genetics
MH  - Homocysteine/blood/*toxicity
MH  - Hydrogen Peroxide/metabolism
MH  - Hydrogen Sulfide/metabolism/pharmacology/*therapeutic use
MH  - Lipid Peroxidation/drug effects
MH  - Male
MH  - Mitochondria, Heart/drug effects/enzymology/metabolism
MH  - Molecular Chaperones/drug effects/genetics
MH  - Myocardium/*metabolism/pathology
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reactive Oxygen Species/antagonists & inhibitors/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Succinate Dehydrogenase/drug effects/metabolism
MH  - Superoxide Dismutase/drug effects/metabolism
MH  - Superoxides/metabolism
MH  - Time Factors
EDAT- 2007/12/12 09:00
MHDA- 2008/08/16 09:00
CRDT- 2007/12/12 09:00
PHST- 2007/10/12 00:00 [received]
PHST- 2007/11/17 00:00 [accepted]
PHST- 2007/12/12 09:00 [pubmed]
PHST- 2008/08/16 09:00 [medline]
PHST- 2007/12/12 09:00 [entrez]
AID - 10.1007/s00726-007-0011-8 [doi]
PST - ppublish
SO  - Amino Acids. 2008 May;34(4):573-85. doi: 10.1007/s00726-007-0011-8. Epub 2007 Dec 
      11.