PMID- 18071850
OWN - NLM
STAT- MEDLINE
DCOM- 20080513
LR  - 20211020
IS  - 1434-7229 (Print)
IS  - 1434-7229 (Linking)
VI  - 10
IP  - 4
DP  - 2007
TI  - Acceleration of bone formation with BMP2 in frame-reinforced carbonate 
      apatite-collagen sponge scaffolds.
PG  - 212-7
AB  - The development is expected of scaffold biomaterials that feature a 
      shape-maintaining property in addition to high porosity and large pores that 
      cells can easily invade. To develop a new biodegradable scaffold biomaterial 
      reinforced with a frame, synthesized carbonate apatite (CO3Ap) was mixed with 
      neutralized collagen gel, and the CO3Ap-collagen mixtures were lyophilized into 
      sponges in a porous hydroxyapatite (HAp) frame ring. X-ray diffraction and 
      Fourier transform infrared spectroscopy (FT-IR) analyses together with chemical 
      analysis indicated that the synthesized CO3Ap had a crystalline nature and a 
      chemical composition similar to that of bone. Scanning electron microscope (SEM) 
      observation showed that the CO3Ap-collagen sponge had a sui pore size for cell 
      invasion. In proliferation and differentiation experiments with osteoblasts, 
      alkaline phosphatase and osteopontin activity were clearly detected. When these 
      sponge-frame complexes with bone morphogenic protein (rh-BMP2) were implanted 
      beneath the periosteum cranii of rats, significant new bone was created at the 
      surface of the periosteum cranii after 4 weeks of implantation. These reinforced 
      CO3Ap-collagen sponges with rh-BMP2 are expected to be used as hard tissue 
      scaffold biomaterials for the therapeutic purpose of the rapid cure of bone 
      defects.
FAU - Hirata, Isao
AU  - Hirata I
AD  - Department of Biomaterials Science, Graduate School of Biomedical Sciences, 
      Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan.
FAU - Nomura, Yuji
AU  - Nomura Y
FAU - Ito, Manabu
AU  - Ito M
FAU - Shimazu, Atsushi
AU  - Shimazu A
FAU - Okazaki, Masayuki
AU  - Okazaki M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071220
PL  - Japan
TA  - J Artif Organs
JT  - Journal of artificial organs : the official journal of the Japanese Society for 
      Artificial Organs
JID - 9815648
RN  - 0 (Apatites)
RN  - 0 (BMP2 protein, human)
RN  - 0 (Bmp2 protein, mouse)
RN  - 0 (Bmp2 protein, rat)
RN  - 0 (Bone Morphogenetic Protein 2)
RN  - 0 (Bone Morphogenetic Proteins)
RN  - 0 (Carbonates)
RN  - 0 (Gels)
RN  - 0 (Transforming Growth Factor beta)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - *Absorbable Implants
MH  - Animals
MH  - Apatites/*chemistry
MH  - Bone Morphogenetic Protein 2
MH  - Bone Morphogenetic Proteins/*pharmacology
MH  - Carbonates/*chemistry
MH  - Cattle
MH  - Cell Culture Techniques
MH  - Collagen/*chemistry
MH  - Gels
MH  - Humans
MH  - Male
MH  - Mice
MH  - Microscopy, Electron, Scanning
MH  - *Osteogenesis
MH  - Porosity
MH  - Rats
MH  - Rats, Wistar
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Spectroscopy, Fourier Transform Infrared
MH  - *Tissue Engineering
MH  - Transforming Growth Factor beta/*pharmacology
MH  - X-Ray Diffraction
EDAT- 2007/12/12 09:00
MHDA- 2008/05/14 09:00
CRDT- 2007/12/12 09:00
PHST- 2007/03/25 00:00 [received]
PHST- 2007/06/19 00:00 [accepted]
PHST- 2007/12/12 09:00 [pubmed]
PHST- 2008/05/14 09:00 [medline]
PHST- 2007/12/12 09:00 [entrez]
AID - 10.1007/s10047-007-0391-2 [doi]
PST - ppublish
SO  - J Artif Organs. 2007;10(4):212-7. doi: 10.1007/s10047-007-0391-2. Epub 2007 Dec 
      20.