PMID- 18072224
OWN - NLM
STAT- MEDLINE
DCOM- 20080401
LR  - 20071217
IS  - 0173-0835 (Print)
IS  - 0173-0835 (Linking)
VI  - 28
IP  - 24
DP  - 2007 Dec
TI  - Channel wall coating on a poly-(methyl methacrylate) CE microchip by thermal 
      immobilization of a cellulose derivative for size-based protein separation.
PG  - 4582-9
AB  - We demonstrate channel wall coating using a cellulose derivative on a 
      poly-(methyl methacrylate) (PMMA) CE microchip to eliminate EOF disturbing 
      protein separation. The channel walls were modified by preconditioning with a 
      solution containing the cellulose derivative and then thermally evaporating the 
      solution to produce hydrophilic channel walls which prevent adsorption of 
      analytes via a hydrophobic interaction. When the PMMA substrate was coated with 
      the cellulose derivative hydroxypropylmethylcellulose (HPMC) 90SH, the water 
      contact angle on the coated substrate was decreased (up to 15 degrees ) and EOF 
      was significantly suppressed (up to 4.0 x 10(-6) cm2.V(-1)s(-1)). Three proteins 
      (20.5, 68.0, and 114.6 kDa) were successfully separated on the 0.15% HPMC 
      90SH-coated channel walls with good reproducibility of migration time (RSD 
      <1.75%) and high efficiency (theoretical plate number per meter: 2.62 x 10(5)).
FAU - Okada, Hiroki
AU  - Okada H
AD  - Department of Applied Chemistry, Graduate School of Engineering, Nagoya 
      University, Nagoya, Japan. h051102d@mbox.nagoya-u.ac.jp
FAU - Kaji, Noritada
AU  - Kaji N
FAU - Tokeshi, Manabu
AU  - Tokeshi M
FAU - Baba, Yoshinobu
AU  - Baba Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Electrophoresis
JT  - Electrophoresis
JID - 8204476
RN  - 0 (Proteins)
RN  - 0 (Solutions)
RN  - 9004-34-6 (Cellulose)
RN  - 9011-14-7 (Polymethyl Methacrylate)
SB  - IM
MH  - Cellulose/*chemistry
MH  - Electrophoresis, Capillary/*instrumentation
MH  - Polymethyl Methacrylate/*chemistry
MH  - Proteins/*isolation & purification
MH  - Solutions
MH  - Stereoisomerism
EDAT- 2007/12/12 09:00
MHDA- 2008/04/02 09:00
CRDT- 2007/12/12 09:00
PHST- 2007/12/12 09:00 [pubmed]
PHST- 2008/04/02 09:00 [medline]
PHST- 2007/12/12 09:00 [entrez]
AID - 10.1002/elps.200700105 [doi]
PST - ppublish
SO  - Electrophoresis. 2007 Dec;28(24):4582-9. doi: 10.1002/elps.200700105.