PMID- 18073212
OWN - NLM
STAT- MEDLINE
DCOM- 20080410
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 283
IP  - 8
DP  - 2008 Feb 22
TI  - Structural and biophysical analysis of the DNA binding properties of myelin 
      transcription factor 1.
PG  - 5158-67
AB  - Zinc binding domains, or zinc fingers (ZnFs), form one of the most numerous and 
      most diverse superclasses of protein structural motifs in eukaryotes. Although 
      our understanding of the functions of several classes of these domains is 
      relatively well developed, we know much less about the molecular mechanisms of 
      action of many others. Myelin transcription factor 1 (MyT1) type ZnFs are found 
      in organisms as diverse as nematodes and mammals and are found in a range of 
      sequence contexts. MyT1, one of the early transcription factors expressed in the 
      developing central nervous system, contains seven MyT1 ZnFs that are very highly 
      conserved both within the protein and between species. We have used a range of 
      biophysical techniques, including NMR spectroscopy and data-driven macromolecular 
      docking, to investigate the structural basis for the interaction between MyT1 
      ZnFs and DNA. Our data indicate that MyT1 ZnFs recognize the major groove of DNA 
      in a way that appears to differ from other known zinc binding domains.
FAU - Gamsjaeger, Roland
AU  - Gamsjaeger R
AD  - School of Molecular and Microbial Biosciences, University of Sydney, Building 
      G08, New South Wales, Sydney 2006, Australia.
FAU - Swanton, Michael K
AU  - Swanton MK
FAU - Kobus, Felix J
AU  - Kobus FJ
FAU - Lehtomaki, Eija
AU  - Lehtomaki E
FAU - Lowry, Jason A
AU  - Lowry JA
FAU - Kwan, Ann H
AU  - Kwan AH
FAU - Matthews, Jacqueline M
AU  - Matthews JM
FAU - Mackay, Joel P
AU  - Mackay JP
LA  - eng
SI  - PDB/2JX1
SI  - PDB/2JYD
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071211
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Myt1 protein, mouse)
RN  - 0 (Transcription Factors)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Central Nervous System/embryology
MH  - DNA/*chemistry/metabolism
MH  - DNA-Binding Proteins/*chemistry/metabolism
MH  - Mice
MH  - *Models, Molecular
MH  - Nematoda/embryology
MH  - Nuclear Magnetic Resonance, Biomolecular
MH  - Protein Binding/physiology
MH  - Structure-Activity Relationship
MH  - Transcription Factors/*chemistry/metabolism
MH  - Zinc Fingers/*physiology
EDAT- 2007/12/13 09:00
MHDA- 2008/04/11 09:00
CRDT- 2007/12/13 09:00
PHST- 2007/12/13 09:00 [pubmed]
PHST- 2008/04/11 09:00 [medline]
PHST- 2007/12/13 09:00 [entrez]
AID - S0021-9258(20)68277-1 [pii]
AID - 10.1074/jbc.M703772200 [doi]
PST - ppublish
SO  - J Biol Chem. 2008 Feb 22;283(8):5158-67. doi: 10.1074/jbc.M703772200. Epub 2007 
      Dec 11.