PMID- 18073319
OWN - NLM
STAT- MEDLINE
DCOM- 20080311
LR  - 20220311
IS  - 0193-1849 (Print)
IS  - 0193-1849 (Linking)
VI  - 294
IP  - 2
DP  - 2008 Feb
TI  - PGC-1alpha is not mandatory for exercise- and training-induced adaptive gene 
      responses in mouse skeletal muscle.
PG  - E463-74
AB  - The aim of the present study was to test the hypothesis that peroxisome 
      proliferator activated receptor-gamma coactivator (PGC) 1alpha is required for 
      exercise-induced adaptive gene responses in skeletal muscle. Whole body 
      PGC-1alpha knockout (KO) and littermate wild-type (WT) mice performed a single 
      treadmill-running exercise bout. Soleus and white gastrocnemius (WG) were 
      obtained immediately, 2 h, or 6 h after exercise. Another group of PGC-1alpha KO 
      and WT mice performed 5-wk exercise training. Soleus, WG, and quadriceps were 
      obtained approximately 37 h after the last training session. Resting muscles of 
      the PGC-1alpha KO mice had lower ( approximately 20%) cytochrome c (cyt c), 
      cytochrome oxidase (COX) I, and aminolevulinate synthase (ALAS) 1 mRNA and 
      protein levels than WT, but similar levels of AMP-activated protein kinase (AMPK) 
      alpha1, AMPKalpha2, and hexokinase (HK) II compared with WT mice. A single 
      exercise bout increased phosphorylation of AMPK and acetyl-CoA carboxylase-beta 
      and the level of HKII mRNA similarly in WG of KO and WT. In contrast, cyt c mRNA 
      in soleus was upregulated in WT muscles only. Exercise training increased cyt c, 
      COXI, ALAS1, and HKII mRNA and protein levels equally in WT and KO animals, but 
      cyt c, COXI, and ALAS1 expression remained approximately 20% lower in KO animals. 
      In conclusion, lack of PGC-1alpha reduced resting expression of cyt c, COXI, and 
      ALAS1 and exercise-induced cyt c mRNA expression. However, PGC-1alpha is not 
      mandatory for training-induced increases in ALAS1, COXI, and cyt c expression, 
      showing that factors other than PGC-1alpha can exert these adaptations.
FAU - Leick, Lotte
AU  - Leick L
AD  - Centre of Inflammation and Metabolism, and Copenhagen Muscle Research Centre, 
      Department of Molecular Biology, Institute of Exercise and Sport Sciences, 
      University of Copenhagen, Universitetsparken 13, Copenhagen, Denmark. 
      lleick@aki.ku.dk
FAU - Wojtaszewski, Jorgen F P
AU  - Wojtaszewski JF
FAU - Johansen, Sune T
AU  - Johansen ST
FAU - Kiilerich, Kristian
AU  - Kiilerich K
FAU - Comes, Gemma
AU  - Comes G
FAU - Hellsten, Ylva
AU  - Hellsten Y
FAU - Hidalgo, Juan
AU  - Hidalgo J
FAU - Pilegaard, Henriette
AU  - Pilegaard H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071211
PL  - United States
TA  - Am J Physiol Endocrinol Metab
JT  - American journal of physiology. Endocrinology and metabolism
JID - 100901226
RN  - 0 (Muscle Proteins)
RN  - 0 (Myoglobin)
RN  - 0 (Nucleotides)
RN  - 0 (Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha)
RN  - 0 (Ppargc1a protein, mouse)
RN  - 0 (RNA, Messenger)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 9005-79-2 (Glycogen)
RN  - 9007-43-6 (Cytochromes c)
RN  - EC 1.14.99.1 (Cyclooxygenase 1)
RN  - EC 2.3.1.37 (5-Aminolevulinate Synthetase)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
SB  - IM
MH  - 5-Aminolevulinate Synthetase/metabolism
MH  - Adaptation, Physiological/*genetics/*physiology
MH  - Animals
MH  - Blotting, Western
MH  - Body Weight/physiology
MH  - Cyclic AMP-Dependent Protein Kinases/metabolism
MH  - Cyclooxygenase 1/metabolism
MH  - Cytochromes c/biosynthesis/genetics
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Gene Expression Regulation/*physiology
MH  - Glycogen/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Mitochondria, Muscle/metabolism
MH  - Muscle Proteins/biosynthesis
MH  - Muscle, Skeletal/*physiology
MH  - Myoglobin/metabolism
MH  - Nucleotides/metabolism
MH  - Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
MH  - Physical Conditioning, Animal/*physiology
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Trans-Activators/*genetics/*physiology
MH  - Transcription Factors
EDAT- 2007/12/13 09:00
MHDA- 2008/03/12 09:00
CRDT- 2007/12/13 09:00
PHST- 2007/12/13 09:00 [pubmed]
PHST- 2008/03/12 09:00 [medline]
PHST- 2007/12/13 09:00 [entrez]
AID - 00666.2007 [pii]
AID - 10.1152/ajpendo.00666.2007 [doi]
PST - ppublish
SO  - Am J Physiol Endocrinol Metab. 2008 Feb;294(2):E463-74. doi: 
      10.1152/ajpendo.00666.2007. Epub 2007 Dec 11.