PMID- 18073477
OWN - NLM
STAT- MEDLINE
DCOM- 20080710
LR  - 20080630
IS  - 1421-9751 (Electronic)
IS  - 0008-6312 (Linking)
VI  - 110
IP  - 4
DP  - 2008
TI  - Effects of chronic therapy with cardiac contractility modulation electrical 
      signals on cytoskeletal proteins and matrix metalloproteinases in dogs with heart 
      failure.
PG  - 230-7
AB  - OBJECTIVES: Therapy with cardiac contractility modulation (CCM) electrical 
      signals delivered to left ventricular (LV) muscle during the absolute refractory 
      period improves LV systolic and diastolic function in dogs with heart failure 
      (HF). This study examined the effects of CCM therapy on mRNA and protein 
      expression of cytoskeletal proteins, matrix metalloproteinases (MMPs) and tissue 
      inhibitors of MMPs (TIMPs) in the LV myocardium of dogs with HF. METHODS: HF was 
      produced in 14 dogs by coronary microembolizations. Dogs were randomized to 3 
      months of CCM therapy (n = 7) or to sham-operated controls (n = 7). LV tissue 
      from 6 normal (NL) dogs was used for comparison. mRNA expression was measured 
      using reverse-transcriptase polymerase chain reaction and protein expression 
      using Western blots. RESULTS: Compared with NL dogs, controls showed upregulation 
      of mRNA and protein expression of the cytoskeletal proteins tubulin and 
      fibronectin and MMP-1, MMP-2 and MMP-9, and downregulation of the cytoskeletal 
      protein titin. Normalized expression of all these genes and proteins was seen 
      after CCM therapy. No differences in expression of TIMP-1 and TIMP-2 were 
      observed among groups. CONCLUSIONS: CCM therapy normalizes expression of key 
      cytoskeletal proteins and MMPs and may partly explain the improvement in LV 
      function seen in HF following CCM therapy.
CI  - (c) 2007 S. Karger AG, Basel
FAU - Rastogi, Sharad
AU  - Rastogi S
AD  - Division of Cardiovascular Medicine, Henry Ford Heart and Vascular Institute, 
      Detroit, Mich. 48202, USA.
FAU - Mishra, Sudhish
AU  - Mishra S
FAU - Zaca, Valerio
AU  - Zaca V
FAU - Mika, Yuval
AU  - Mika Y
FAU - Rousso, Benny
AU  - Rousso B
FAU - Sabbah, Hani N
AU  - Sabbah HN
LA  - eng
GR  - P01 HL074237-04/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20071212
PL  - Switzerland
TA  - Cardiology
JT  - Cardiology
JID - 1266406
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 127497-59-0 (Tissue Inhibitor of Metalloproteinase-2)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - Animals
MH  - *Cardiac Pacing, Artificial
MH  - Cytoskeletal Proteins/genetics/*metabolism
MH  - Dogs
MH  - Electrophysiology
MH  - Heart Failure/*metabolism/therapy
MH  - Matrix Metalloproteinases/genetics/*metabolism
MH  - Myocardial Contraction
MH  - RNA, Messenger/metabolism
MH  - Tissue Inhibitor of Metalloproteinase-1/genetics/metabolism
MH  - Tissue Inhibitor of Metalloproteinase-2/genetics/metabolism
EDAT- 2007/12/13 09:00
MHDA- 2008/07/11 09:00
CRDT- 2007/12/13 09:00
PHST- 2007/06/27 00:00 [received]
PHST- 2007/07/27 00:00 [accepted]
PHST- 2007/12/13 09:00 [pubmed]
PHST- 2008/07/11 09:00 [medline]
PHST- 2007/12/13 09:00 [entrez]
AID - 000112405 [pii]
AID - 10.1159/000112405 [doi]
PST - ppublish
SO  - Cardiology. 2008;110(4):230-7. doi: 10.1159/000112405. Epub 2007 Dec 12.