PMID- 18074111
OWN - NLM
STAT- MEDLINE
DCOM- 20080620
LR  - 20211020
IS  - 0300-8177 (Print)
IS  - 0300-8177 (Linking)
VI  - 310
IP  - 1-2
DP  - 2008 Mar
TI  - Association of polymorphism in the thermolabile 5, 10-methylene tetrahydrofolate 
      reductase gene and hyperhomocysteinemia with coronary artery disease.
PG  - 111-7
AB  - OBJECTIVE: To determine the incidence of methylene tetrahydrofolate reductase 
      (MTHFR) gene 677C-->T polymorphism and plasma homocysteine (Hcy) levels in a 
      group of subjects who underwent coronary angiography, in an attempt to establish 
      a correlation between these parameters and the severity of coronary artery 
      disease (CAD) and to investigate the correlation between hyperhomocysteinemia 
      (HHcy) and the presence of 677C-->T polymorphism. BACKGROUND: Elevated plasma Hcy 
      level is an independent risk factor for CAD. A common mutation (677C-->T) in the 
      gene coding for MTHFR has been reported to reduce the enzymatic activity and is 
      associated with elevated levels of Hcy, especially in subjects with low folate 
      intake. METHODS: The study group comprised of 84 patients with CAD and 100 
      age-and-sex matched controls who had no history or clinical evidence of CAD 
      and/or MI. DNA was extracted from peripheral blood and genotypes were determined 
      by polymerase chain reaction, restriction mapping with Hinf1, and gel 
      electrophoresis. Conventional risk factors for CAD were prospectively documented. 
      RESULTS: Allele and genotype frequencies in cases and control subjects were 
      compatible with Hardy-Weinberg equilibrium. The frequencies of TT, CT, and CC 
      genotypes among CAD patients were 4.8, 27.4, and 67.8% and in controls were 1.0, 
      19.0, and 80%. Hcy levels were higher in patients with triple-vessel disease 
      compared to single and double vessel disease (P = 0.002). Multivariate analyses 
      identified HHcy, diabetes mellitus, and hypertension as the independent 
      predictors of CAD. CONCLUSIONS: HHcy appears to have a graded effect on the risk 
      of CAD as well as the severity and extent of coronary atherosclerosis. Our 
      findings support that homozygous genotype of MTHFR is a genetic risk factor for 
      CAD. A further study with larger sample size including assessment of vitamin 
      status is needed to better clarify the relationship between MTHFR genotypes and 
      CAD.
FAU - Alam, Mohammad A
AU  - Alam MA
AD  - Department of Paediatrics, All India Institute of Medical Sciences, New Delhi, 
      India.
FAU - Husain, Syed A
AU  - Husain SA
FAU - Narang, Rajiv
AU  - Narang R
FAU - Chauhan, Shayam S
AU  - Chauhan SS
FAU - Kabra, Madhulika
AU  - Kabra M
FAU - Vasisht, Suman
AU  - Vasisht S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071212
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - EC 1.5.1.20 (Methylenetetrahydrofolate Reductase (NADPH2))
SB  - IM
MH  - Coronary Artery Disease/*complications/*enzymology/genetics
MH  - Enzyme Stability
MH  - *Genetic Predisposition to Disease
MH  - Homocysteine/metabolism
MH  - Humans
MH  - Hyperhomocysteinemia/*complications/*enzymology/genetics
MH  - Methylenetetrahydrofolate Reductase (NADPH2)/*genetics
MH  - Middle Aged
MH  - Multivariate Analysis
MH  - Phenotype
MH  - Polymorphism, Single Nucleotide/*genetics
MH  - Regression Analysis
EDAT- 2007/12/13 09:00
MHDA- 2008/06/21 09:00
CRDT- 2007/12/13 09:00
PHST- 2007/05/29 00:00 [received]
PHST- 2007/11/22 00:00 [accepted]
PHST- 2007/12/13 09:00 [pubmed]
PHST- 2008/06/21 09:00 [medline]
PHST- 2007/12/13 09:00 [entrez]
AID - 10.1007/s11010-007-9671-7 [doi]
PST - ppublish
SO  - Mol Cell Biochem. 2008 Mar;310(1-2):111-7. doi: 10.1007/s11010-007-9671-7. Epub 
      2007 Dec 12.