PMID- 18074272
OWN - NLM
STAT- MEDLINE
DCOM- 20080116
LR  - 20220316
IS  - 1473-7760 (Electronic)
IS  - 1052-0295 (Linking)
VI  - 82
IP  - 4-5
DP  - 2007 Aug
TI  - Mapping cynomolgus monkey MHC class I district on chromosome 6p13 using pooled 
      cDNAs.
PG  - 267-72
AB  - The cynomolgus monkey (Macaca fascicularis) is a frequently used animal model for 
      studying human diseases, especially immune related ones. For a better 
      understanding of its major histocompatibility complex (MHC) class I district 
      chromosome location, we selected seven cDNA clones as probes for fluorescence in 
      situ hybridization (FISH) from a lymphocyte cell line cDNA library. Expressed 
      sequence tags (ESTs) from these clones were assembled into three clusters and 
      annotated Mafa-A and Mafa-B genes. Further bioinformatics analysis shows that 
      they had multiple duplications spanning approximately 2.8 Mb on the rhesus 
      macaque MHC class I district. Using the FISH technique, we mapped the seven 
      pooled cDNA clones to the short arm of the cynomolgus monkey chromosome 6 on 
      6p13. To our knowledge, this is the first report of the location of cynomolgus 
      monkey MHC class I district. Using pooled adjacent cDNAs as probes also allows 
      affordable, specific genome region mapping research.
FAU - Liu, Q Y
AU  - Liu QY
AD  - Beijing Institute of Genomics, Beijing, China.
FAU - Wang, X X
AU  - Wang XX
FAU - Zhang, J Z
AU  - Zhang JZ
FAU - Chen, W H
AU  - Chen WH
FAU - He, X W
AU  - He XW
FAU - Lin, Y
AU  - Lin Y
FAU - Wang, J F
AU  - Wang JF
FAU - Zhu, Y
AU  - Zhu Y
FAU - Hu, S N
AU  - Hu SN
FAU - Wang, X N
AU  - Wang XN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biotech Histochem
JT  - Biotechnic & histochemistry : official publication of the Biological Stain 
      Commission
JID - 9107378
SB  - IM
MH  - Animals
MH  - Chromosome Banding/*methods
MH  - Chromosomes, Human, Pair 6/*genetics/ultrastructure
MH  - Contig Mapping/*methods
MH  - *Gene Library
MH  - Genes, MHC Class I/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Macaca fascicularis/*genetics
EDAT- 2007/12/13 09:00
MHDA- 2008/01/17 09:00
CRDT- 2007/12/13 09:00
PHST- 2007/12/13 09:00 [pubmed]
PHST- 2008/01/17 09:00 [medline]
PHST- 2007/12/13 09:00 [entrez]
AID - 784702052 [pii]
AID - 10.1080/10520290701753987 [doi]
PST - ppublish
SO  - Biotech Histochem. 2007 Aug;82(4-5):267-72. doi: 10.1080/10520290701753987.