PMID- 18077699
OWN - NLM
STAT- MEDLINE
DCOM- 20080122
LR  - 20211020
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 27
IP  - 50
DP  - 2007 Dec 12
TI  - Exuberant neuronal convergence onto reduced taste bud targets with preservation 
      of neural specificity in mice overexpressing neurotrophin in the tongue 
      epithelium.
PG  - 13875-81
AB  - A mouse fungiform taste bud is innervated by only four to five geniculate 
      ganglion neurons; their peripheral fibers do not branch to other buds. We 
      examined whether the degree or specificity of this exclusive innervation pattern 
      is influenced by brain-derived neurotrophic factor (BDNF), a prominent lingual 
      neurotrophin implicated in taste receptoneural development. Labeled ganglion 
      cells were counted after injecting single buds with different color markers in 
      BDNF-lingual-overexpressing (OE) mice. To evaluate the end-organs, taste buds and 
      a class of putative taste receptor cells were counted from progeny of BDNF-OE 
      mice crossbred with green fluorescent protein (GFP) (gustducin) transgenic mice. 
      Fungiform bud numbers in BDNF-OE mice are 35%, yet geniculate neuron numbers are 
      195%, of wild-type mice. Neurons labeled by single-bud injections in BDNF-OE 
      animals were increased fourfold versus controls. Injecting three buds, each with 
      different color markers, resulted in predominantly single-labeled ganglion cells, 
      a discrete innervation pattern similar to controls. Thus, hyper-innervation of 
      BDNF-OE buds involves many neurons innervating single buds, not increased fiber 
      branching. Therefore, both wild-type and BDNF-OE mice exhibit, in fungiform buds, 
      the same, "discrete" receptoneural pattern, this despite dramatic neurotrophin 
      overexpression-related decreases in bud numbers and increases in innervation 
      density. Hyperinnervation did not affect GFP positive cell numbers; proportions 
      of GFP cells in BDNF-OE buds were the same as in wild-type mice. Total numbers of 
      ganglion cells innervating buds in transgenic mice are similar to controls; the 
      density of taste input to the brain appears maintained despite dramatically 
      reduced receptor organs and increased ganglion cells.
FAU - Zaidi, Faisal N
AU  - Zaidi FN
AD  - Howard Hughes Medical Institute and Department of Neurobiology, University of 
      California, San Diego, La Jolla, California 92093, USA.
FAU - Krimm, Robin F
AU  - Krimm RF
FAU - Whitehead, Mark C
AU  - Whitehead MC
LA  - eng
GR  - R01 DC007176/DC/NIDCD NIH HHS/United States
GR  - R01 DC01091/DC/NIDCD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Nerve Growth Factors)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Cell Count
MH  - Crosses, Genetic
MH  - Epithelial Cells/*metabolism
MH  - Fluorescent Dyes/pharmacokinetics
MH  - Geniculate Ganglion/abnormalities/pathology
MH  - Green Fluorescent Proteins/biosynthesis/genetics
MH  - Iontophoresis
MH  - Mice
MH  - Mice, Transgenic
MH  - Nerve Growth Factors/*biosynthesis/genetics
MH  - Neurons, Afferent/*metabolism/pathology
MH  - Taste Buds/*abnormalities/*metabolism/pathology
MH  - Tongue/*innervation
PMC - PMC6673623
EDAT- 2007/12/14 09:00
MHDA- 2008/01/23 09:00
PMCR- 2008/06/12
CRDT- 2007/12/14 09:00
PHST- 2007/12/14 09:00 [pubmed]
PHST- 2008/01/23 09:00 [medline]
PHST- 2007/12/14 09:00 [entrez]
PHST- 2008/06/12 00:00 [pmc-release]
AID - 27/50/13875 [pii]
AID - 3299370 [pii]
AID - 10.1523/JNEUROSCI.2517-07.2007 [doi]
PST - ppublish
SO  - J Neurosci. 2007 Dec 12;27(50):13875-81. doi: 10.1523/JNEUROSCI.2517-07.2007.