PMID- 18078768
OWN - NLM
STAT- MEDLINE
DCOM- 20090428
LR  - 20111117
IS  - 1090-0233 (Print)
IS  - 1090-0233 (Linking)
VI  - 180
IP  - 1
DP  - 2009 Apr
TI  - Partial sequencing and expression of genes involved in glucose metabolism in 
      adipose tissues and skeletal muscle of healthy cats.
PG  - 66-70
AB  - Impaired insulin sensitivity is increasingly recognised in cats, but sequences of 
      genes involved in insulin-signalling are largely undetermined in this species. In 
      this study, extended feline mRNA sequences were determined for the adiponectin, 
      glucose transporter-1 (GLUT1), GLUT4, peroxisome proliferative activated 
      receptor-gamma1 (PPARgamma1), PPARgamma2, plasminogen activator inhibitor-1 
      (PAI-1), monocyte chemoattractant protein-1 (MCP-1) and insulin receptor genes. 
      Conserved dog-specific primers identified from human-dog mRNA alignments were 
      used to amplify feline cDNA in the polymerase chain reaction (PCR). The feline 
      sequences determined by this method were used to design feline-specific primers 
      suitable for real-time PCR for quantification of gene expression in insulin 
      sensitive tissues of healthy cats. Partial sequences of feline mRNAs had 86-95% 
      identity with dog and human genes. Expression of adiponectin, GLUT1, GLUT4, 
      PPARgamma1, PPARgamma2, PAI-1 and insulin receptor mRNA was detected and 
      quantified in subcutaneous and visceral fat and skeletal muscle, whereas MCP-1 
      mRNA was detected in adipose tissue but not in skeletal muscle. Further 
      characterisation of genes related to glucose metabolism in cats will provide 
      additional insights into insulin-signalling mechanisms in this species.
FAU - Zini, Eric
AU  - Zini E
AD  - Clinic for Small Animal Internal Medicine, Vetsuisse Faculty, University of 
      Zurich, Winterthurerstr. 260, 8057 Zurich, Switzerland. ezini@vetclinics.uzh.ch
FAU - Linscheid, Philippe
AU  - Linscheid P
FAU - Franchini, Marco
AU  - Franchini M
FAU - Kaufmann, Karin
AU  - Kaufmann K
FAU - Monnais, Edouard
AU  - Monnais E
FAU - Kutter, Annette P
AU  - Kutter AP
FAU - Ackermann, Mathias
AU  - Ackermann M
FAU - Lutz, Thomas A
AU  - Lutz TA
FAU - Reusch, Claudia E
AU  - Reusch CE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071219
PL  - England
TA  - Vet J
JT  - Veterinary journal (London, England : 1997)
JID - 9706281
RN  - 0 (Blood Glucose)
RN  - 0 (Glucose Transport Proteins, Facilitative)
RN  - 0 (Insulin)
RN  - 0 (PPAR gamma)
RN  - EC 2.7.10.1 (Receptor, Insulin)
SB  - IM
MH  - Adipose Tissue/*metabolism
MH  - Animals
MH  - Blood Glucose/*metabolism
MH  - Cats/genetics/*metabolism
MH  - Energy Metabolism/*genetics
MH  - Female
MH  - Gene Expression/genetics
MH  - Glucose Transport Proteins, Facilitative/genetics
MH  - Insulin/blood
MH  - Insulin Resistance/*genetics
MH  - Male
MH  - Muscle, Skeletal/*metabolism
MH  - PPAR gamma/genetics
MH  - Receptor, Insulin/genetics
MH  - Species Specificity
EDAT- 2007/12/15 09:00
MHDA- 2009/04/29 09:00
CRDT- 2007/12/15 09:00
PHST- 2007/08/14 00:00 [received]
PHST- 2007/10/27 00:00 [revised]
PHST- 2007/10/31 00:00 [accepted]
PHST- 2007/12/15 09:00 [pubmed]
PHST- 2009/04/29 09:00 [medline]
PHST- 2007/12/15 09:00 [entrez]
AID - S1090-0233(07)00382-6 [pii]
AID - 10.1016/j.tvjl.2007.10.022 [doi]
PST - ppublish
SO  - Vet J. 2009 Apr;180(1):66-70. doi: 10.1016/j.tvjl.2007.10.022. Epub 2007 Dec 19.