PMID- 18080182
OWN - NLM
STAT- MEDLINE
DCOM- 20080624
LR  - 20220716
IS  - 0282-0080 (Print)
IS  - 0282-0080 (Linking)
VI  - 25
IP  - 3
DP  - 2008 Apr
TI  - Surface alpha 2-3- and alpha 2-6-sialylation of human monocytes and derived 
      dendritic cells and its influence on endocytosis.
PG  - 259-68
AB  - Several glycoconjugates are involved in the immune response. Sialic acid is 
      frequently the glycan terminal sugar and it may modulate immune interactions. 
      Dendritic cells (DCs) are antigen-presenting cells with high endocytic capacity 
      and a central role in immune regulation. On this basis, DCs derived from 
      monocytes (mo-DC) are utilised in immunotherapy, though many features are ignored 
      and their use is still limited. We analyzed the surface sialylated glycans 
      expressed during human mo-DC generation. This was monitored by lectin binding and 
      analysis of sialyltransferases (ST) at the mRNA level and by specific enzymatic 
      assays. We showed that alpha 2-3-sialylated O-glycans and alpha 2-6- and alpha 
      2-3-sialylated N-glycans are present in monocytes and their expression increases 
      during mo-DC differentiation. Three main ST genes are committed with this 
      rearrangement: ST6Gal1 is specifically involved in the augmented alpha 
      2-6-sialylated N-glycans; ST3Gal1 contributes for the alpha2-3-sialylation of 
      O-glycans, particularly T antigens; and ST3Gal4 may contribute for the increased 
      alpha2-3-sialylated N-glycans. Upon mo-DC maturation, ST6Gal1 and ST3Gal4 are 
      downregulated and ST3Gal1 is altered in a stimulus-dependent manner. We also 
      observed that removing surface sialic acid of immature mo-DC by neuraminidase 
      significantly decreased its endocytic capacity, while it increased in monocytes. 
      Our results indicate the STs expression modulates the increased expression of 
      surface sialylated structures during mo-DC generation, which is probably related 
      with changes in cell mechanisms. The ST downregulation after mo-DC maturation 
      probably results in a decreased sialylation or sialylated glycoconjugates 
      involved in the endocytosis, contributing to the downregulation of one or more 
      antigen-uptake mechanisms specific of mo-DC.
FAU - Videira, Paula A
AU  - Videira PA
AD  - Departamento de Imunologia FCM-UNL, Campo Martires da Patria 130, 1169-056,, 
      Lisbon, Portugal. pvideira.imuno@fcm.unl.pt
FAU - Amado, Ines F
AU  - Amado IF
FAU - Crespo, Helio J
AU  - Crespo HJ
FAU - Alguero, M Carmen
AU  - Alguero MC
FAU - Dall'Olio, Fabio
AU  - Dall'Olio F
FAU - Cabral, M Guadalupe
AU  - Cabral MG
FAU - Trindade, Helder
AU  - Trindade H
LA  - eng
PT  - Journal Article
DEP - 20071213
PL  - United States
TA  - Glycoconj J
JT  - Glycoconjugate journal
JID - 8603310
RN  - EC 2.4.99.- (Sialyltransferases)
RN  - GZP2782OP0 (N-Acetylneuraminic Acid)
SB  - IM
MH  - Cell Differentiation
MH  - Cell Membrane/*metabolism
MH  - Cells, Cultured
MH  - Dendritic Cells/*cytology/*metabolism
MH  - *Endocytosis
MH  - Gene Expression Regulation, Enzymologic
MH  - Humans
MH  - Monocytes/*cytology/*metabolism
MH  - N-Acetylneuraminic Acid/*metabolism
MH  - Sialyltransferases/genetics/metabolism
EDAT- 2007/12/15 09:00
MHDA- 2008/06/25 09:00
CRDT- 2007/12/15 09:00
PHST- 2007/03/19 00:00 [received]
PHST- 2007/11/15 00:00 [accepted]
PHST- 2007/08/24 00:00 [revised]
PHST- 2007/12/15 09:00 [pubmed]
PHST- 2008/06/25 09:00 [medline]
PHST- 2007/12/15 09:00 [entrez]
AID - 10.1007/s10719-007-9092-6 [doi]
PST - ppublish
SO  - Glycoconj J. 2008 Apr;25(3):259-68. doi: 10.1007/s10719-007-9092-6. Epub 2007 Dec 
      13.