PMID- 18082569
OWN - NLM
STAT- MEDLINE
DCOM- 20080227
LR  - 20210103
IS  - 0198-8859 (Print)
IS  - 0198-8859 (Linking)
VI  - 68
IP  - 11
DP  - 2007 Nov
TI  - Association of Toll-like receptor-4 (Asp299Gly and Thr399Ileu) gene polymorphisms 
      with gastritis and precancerous lesions.
PG  - 901-7
AB  - A Toll-like receptor-4 (TLR-4) Asp299Gly and Thr399Ileu substitution reduces 
      responsiveness to Helicobacter pylori (H. pylori) lipopolysaccharide. CagA+ 
      strains of H. pylori are known to be associated with gastroduodenal diseases. 
      Therefore we aimed to evaluate association of TLR-4 substitutions and CagA 
      seropositivity with gastritis and precancerous lesions in a northern Indian 
      population. After upper gastrointestinal endoscopy, 130 rapid urease test 
      (RUT)-positive patients with nonulcer dyspepsia (NUD) were included. Patients 
      with NUD were also screened for H. pylori infection using modified Giemsa 
      staining and anti-CagA IgG enzyme-linked immunoabsorbent assay. All patients and 
      200 asymptomatic control subjects were genotyped for TLR-4 substitutions using 
      polymerase chain reaction-restriction fragment length polymorphism. We observed 
      that frequencies of TLR-4 Asp299Gly variants were comparable between patients and 
      control subjects, and also between positive and negative groups of precancerous 
      lesions in patients. Frequencies of TLR-4 399Ileu allele (8% vs 3%, p = 0.008) 
      and Asp299-Ileu399 haplotype (6.5% vs 3%, p = 0.022) were higher in patients than 
      in control subjects at risk for gastritis (OR = 2.6 and 2.5, respectively). TLR-4 
      399Ileu allele carriers had higher risk for plasma cell infiltration (p = 0.023, 
      OR = 10.6) that led to atrophy (p = 0.028, OR = 4.2) and intestinal metaplasia (p 
      = 0.009, OR = 4.7). CagA positivity was more frequently associated with lymphoid 
      follicle formation (p = 0.033, OR = 2.53). In conclusion TLR-4 Thr399Ileu 
      substitution may be a risk factor for gastritis and precancerous lesions. CagA 
      positivity may be a risk factor for lymphoid follicle development but not for 
      other precancerous lesions in a northern Indian population.
FAU - Achyut, B R
AU  - Achyut BR
AD  - Department of Genetics, Sanjay Gandhi Postgraduate Institute of Medical Sciences, 
      Lucknow, India.
FAU - Ghoshal, Uday C
AU  - Ghoshal UC
FAU - Moorchung, Nikhil
AU  - Moorchung N
FAU - Mittal, Balraj
AU  - Mittal B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071105
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Bacterial Proteins)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (cagA protein, Helicobacter pylori)
SB  - IM
MH  - Adult
MH  - Alleles
MH  - Antibodies, Bacterial/*blood
MH  - Antigens, Bacterial/*immunology
MH  - Bacterial Proteins/*immunology
MH  - Female
MH  - Gastritis/*genetics/immunology/metabolism/microbiology
MH  - Gene Frequency
MH  - Genetic Predisposition to Disease
MH  - Genotype
MH  - Helicobacter Infections/immunology/microbiology
MH  - Helicobacter pylori/immunology
MH  - Humans
MH  - India
MH  - Male
MH  - Middle Aged
MH  - *Polymorphism, Genetic
MH  - Polymorphism, Restriction Fragment Length
MH  - Precancerous Conditions/*genetics/immunology/metabolism/microbiology
MH  - Stomach Neoplasms/*genetics/immunology/metabolism/microbiology
MH  - Toll-Like Receptor 4/blood/*genetics
EDAT- 2007/12/18 09:00
MHDA- 2008/02/28 09:00
CRDT- 2007/12/18 09:00
PHST- 2007/07/01 00:00 [received]
PHST- 2007/09/20 00:00 [revised]
PHST- 2007/10/05 00:00 [accepted]
PHST- 2007/12/18 09:00 [pubmed]
PHST- 2008/02/28 09:00 [medline]
PHST- 2007/12/18 09:00 [entrez]
AID - S0198-8859(07)00458-2 [pii]
AID - 10.1016/j.humimm.2007.10.006 [doi]
PST - ppublish
SO  - Hum Immunol. 2007 Nov;68(11):901-7. doi: 10.1016/j.humimm.2007.10.006. Epub 2007 
      Nov 5.