PMID- 18082572
OWN - NLM
STAT- MEDLINE
DCOM- 20080227
LR  - 20111117
IS  - 0198-8859 (Print)
IS  - 0198-8859 (Linking)
VI  - 68
IP  - 11
DP  - 2007 Nov
TI  - High-throughput sequence-based typing strategy for HLA-DRB1 based on real-time 
      polymerase chain reaction.
PG  - 915-7
AB  - DNA sequencing is the gold standard for high-resolution genotyping of the highly 
      polymorphic human leukocyte antigen (HLA) loci. In the case of hematopoietic stem 
      cell transplantation, four-digit typing of HLA class I and II genes is indicated. 
      We developed a group-specific, real-time polymerase chain reaction (PCR) strategy 
      for amplification of DRB1 applying TaqMan chemistry on different real-time 
      machines. For evaluation of genotyping accuracy and ambiguity resolution, 115 
      well-characterized samples were adapted. Separate analysis of each allele was 
      possible in 100 samples (87%). Of the samples, 13% (n = 15) showed amplification 
      in one of the eight group-specific PCR mixes: one sample according to the group 
      DRB1*15, 16, along with 14 samples according to the group DRB1*03, *11, *13, *14. 
      Further testing of the 14 (12.2%) samples associated with group DRB1*03, *11, 
      *13, *14 was performed with specific intron primers. In conclusion this typing 
      scheme generates results within 1 day, thereby making sequencing for different 
      requirements attractive.
FAU - Danzer, Martin
AU  - Danzer M
AD  - Red Cross Transfusion Service of Upper Austria, Linz, Austria. 
      martin.danzer@blutz.o.redcross.or.at
FAU - Polin, Helene
AU  - Polin H
FAU - Proll, Johannes
AU  - Proll J
FAU - Hofer, Katja
AU  - Hofer K
FAU - Fae, Ingrid
AU  - Fae I
FAU - Fischer, Gottfried F
AU  - Fischer GF
FAU - Gabriel, Christian
AU  - Gabriel C
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20071031
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (HLA-DR Antigens)
RN  - 0 (HLA-DRB1 Chains)
SB  - IM
MH  - Alleles
MH  - Base Sequence
MH  - HLA-DR Antigens/*genetics
MH  - HLA-DRB1 Chains
MH  - Humans
MH  - Polymerase Chain Reaction/*methods
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
EDAT- 2007/12/18 09:00
MHDA- 2008/02/28 09:00
CRDT- 2007/12/18 09:00
PHST- 2007/07/31 00:00 [received]
PHST- 2007/09/28 00:00 [revised]
PHST- 2007/10/05 00:00 [accepted]
PHST- 2007/12/18 09:00 [pubmed]
PHST- 2008/02/28 09:00 [medline]
PHST- 2007/12/18 09:00 [entrez]
AID - S0198-8859(07)00457-0 [pii]
AID - 10.1016/j.humimm.2007.10.005 [doi]
PST - ppublish
SO  - Hum Immunol. 2007 Nov;68(11):915-7. doi: 10.1016/j.humimm.2007.10.005. Epub 2007 
      Oct 31.