PMID- 18082866
OWN - NLM
STAT- MEDLINE
DCOM- 20080528
LR  - 20131121
IS  - 0045-6535 (Print)
IS  - 0045-6535 (Linking)
VI  - 71
IP  - 4
DP  - 2008 Mar
TI  - Application of fluorescence in situ hybridization technique to detect 
      simazine-degrading bacteria in soil samples.
PG  - 703-10
AB  - We propose a new approach to evaluate the natural attenuation capacity of soil by 
      using fluorescence in situ hybridization (FISH). A specific oligonucleotide probe 
      AtzB1 was designed based on the sequence data of the atzB gene involved in the 
      hydrolytic deamination of s-triazines; this gene, located in a multiple copy 
      plasmid was detected by the optimized FISH protocol. Two agricultural soils (Lodi 
      and Henares) with a history of simazine treatments, and two natural soils (Soto 
      and Monza), without previous exposure to simazine, were studied. AtzB1 
      probe-target cells were found only in the agricultural soils and, in a greater 
      percentage, in the Lodi soil, compared to the Henares one. Moreover, the greatest 
      percentage of AtzB1 probe-target cells in Lodi was accompanied by a greater 
      mineralization rate, compared to the Henares soil. The FISH method used in this 
      study was suitable for the detection of simazine-degrading bacteria and could be 
      a useful indicator of the potential of soil bioremediation.
FAU - Martin, Margarita
AU  - Martin M
AD  - Complutense University, Avenida Puerta de Hierro s/n, 28040 Madrid, Spain. 
      margamar@vet.ucm.es
FAU - Gibello, Alicia
AU  - Gibello A
FAU - Lobo, Carmen
AU  - Lobo C
FAU - Nande, Mar
AU  - Nande M
FAU - Garbi, Carlos
AU  - Garbi C
FAU - Fajardo, Carmen
AU  - Fajardo C
FAU - Barra-Caracciolo, Anna
AU  - Barra-Caracciolo A
FAU - Grenni, Paola
AU  - Grenni P
FAU - Martinez-Inigo, M Jose
AU  - Martinez-Inigo MJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071220
PL  - England
TA  - Chemosphere
JT  - Chemosphere
JID - 0320657
RN  - 0 (DNA Probes)
RN  - SG0C34SMY3 (Simazine)
SB  - IM
MH  - Agriculture
MH  - Bacteria/*isolation & purification/*metabolism
MH  - *Biodegradation, Environmental
MH  - DNA Probes
MH  - *In Situ Hybridization, Fluorescence
MH  - Polymerase Chain Reaction
MH  - Simazine/*metabolism
MH  - *Soil Microbiology
EDAT- 2007/12/18 09:00
MHDA- 2008/05/29 09:00
CRDT- 2007/12/18 09:00
PHST- 2007/04/23 00:00 [received]
PHST- 2007/10/19 00:00 [revised]
PHST- 2007/10/26 00:00 [accepted]
PHST- 2007/12/18 09:00 [pubmed]
PHST- 2008/05/29 09:00 [medline]
PHST- 2007/12/18 09:00 [entrez]
AID - S0045-6535(07)01351-3 [pii]
AID - 10.1016/j.chemosphere.2007.10.071 [doi]
PST - ppublish
SO  - Chemosphere. 2008 Mar;71(4):703-10. doi: 10.1016/j.chemosphere.2007.10.071. Epub 
      2007 Dec 20.