PMID- 18083239
OWN - NLM
STAT- MEDLINE
DCOM- 20080403
LR  - 20080114
IS  - 0165-2478 (Print)
IS  - 0165-2478 (Linking)
VI  - 115
IP  - 2
DP  - 2008 Jan 29
TI  - Analysis of capturing skin antigens in the steady state using milk fat globule 
      EGF factor 8-deficient skin-hyperpigmented mice.
PG  - 131-7
AB  - Dendritic cells (DCs) can capture apoptotic cells and present them to immune 
      competent cells as self-antigens (Ags). Langerhans cells (LCs), DCs in the 
      epidermis, are capable of presenting tissue-associated Ags in the steady state, 
      suggesting that LCs may also capture apoptotic cells and transport them to skin 
      regional lymph nodes (LNs). However, to what extent LCs utilize apoptotic cells 
      as self-Ags in vivo is still unclear. To clarify this point, we examined the 
      contribution of milk fat globule EGF factor 8 (MFG-E8), a secreted glycoprotein, 
      to capturing skin Ags. MFG-E8 is expressed in several subsets of macrophages (M 
      phi s) and DCs, including LCs, and crucial for recognizing and engulfing 
      apoptotic cells. Using a skin-hyperpigmented KRT14-Kitl-Tg (Kitl-Tg) mouse 
      system, we measured the accumulation of melanin granules (MGs), a marker of skin 
      Ags, transported from the skin to regional LNs in Mfge8-deficient mice. 
      Unexpectedly, their accumulation in Mfge8-deficient Kitl-Tg mice was comparable 
      to that in Mfge8-heterozygous littermates. Mfge8-deficient DCs engulfed 
      skin-derived MGs efficiently in vitro. The results indicate that MFG-E8 does not 
      contribute critically or functions redundantly to capturing and trafficking of 
      skin Ags in the steady state, and suggest a possibility that LCs may capture skin 
      Ags in forms other than apoptotic cells in vivo.
FAU - Yoshino, Miya
AU  - Yoshino M
AD  - Division of Immunology, Department of Molecular and Cellular Biology, School of 
      Life Science, Faculty of Medicine, Tottori University, 86 Nishi-Cho, Yonago 
      683-8503, Japan. myoshi@grape.med.tottori-u.ac.jp
FAU - Yamazaki, Hidetoshi
AU  - Yamazaki H
FAU - Hayashi, Shin-Ichi
AU  - Hayashi S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071203
PL  - Netherlands
TA  - Immunol Lett
JT  - Immunology letters
JID - 7910006
RN  - 0 (Antigens, Surface)
RN  - 0 (Melanins)
RN  - 0 (Mfge8 protein, mouse)
RN  - 0 (Milk Proteins)
SB  - IM
MH  - Animals
MH  - Antigens, Surface/genetics/*metabolism
MH  - *Apoptosis
MH  - Cytoplasmic Granules/immunology
MH  - Dendritic Cells/cytology/immunology/metabolism
MH  - Langerhans Cells/cytology/*immunology/metabolism
MH  - Macrophages/cytology/immunology/metabolism
MH  - Melanins/immunology
MH  - Melanosomes/immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Mutant Strains
MH  - Mice, Transgenic
MH  - Milk Proteins/genetics/*metabolism
MH  - Skin/*immunology
MH  - Skin Pigmentation
EDAT- 2007/12/18 09:00
MHDA- 2008/04/04 09:00
CRDT- 2007/12/18 09:00
PHST- 2007/08/29 00:00 [received]
PHST- 2007/10/22 00:00 [revised]
PHST- 2007/10/28 00:00 [accepted]
PHST- 2007/12/18 09:00 [pubmed]
PHST- 2008/04/04 09:00 [medline]
PHST- 2007/12/18 09:00 [entrez]
AID - S0165-2478(07)00284-2 [pii]
AID - 10.1016/j.imlet.2007.10.017 [doi]
PST - ppublish
SO  - Immunol Lett. 2008 Jan 29;115(2):131-7. doi: 10.1016/j.imlet.2007.10.017. Epub 
      2007 Dec 3.