PMID- 18084608
OWN - NLM
STAT- MEDLINE
DCOM- 20080225
LR  - 20211020
IS  - 1476-5586 (Electronic)
IS  - 1522-8002 (Print)
IS  - 1476-5586 (Linking)
VI  - 9
IP  - 12
DP  - 2007 Dec
TI  - RNA interference of interferon regulatory factor-1 gene expression in THP-1 cell 
      line leads to Toll-like receptor-4 overexpression/activation as well as 
      up-modulation of annexin-II.
PG  - 1012-20
AB  - Interferon regulatory factor-1 (IRF-1) is a candidate transcription factor for 
      the regulation of the Toll-like receptor-4 (TLR-4) gene. Using a small 
      interfering RNA-based (siRNA) process to silence IRF-1 gene expression in the 
      leukemic monocytic cell line THP-1, we investigated whether such a modulation 
      would alter TLR-4 expression and activation status in these cells. The siIRF-1 
      cells expressed elevated levels of TLR-4 mRNA and protein compared to controls by 
      90% and 77%, respectively. ICAM.1 protein expression and apoptosis levels were 
      increased by 8.35- and 4.25-fold, respectively. The siIRF-1 cells overexpressed 
      Bax mRNA compared to controls. Proteomic analysis revealed upmodulation of the 
      Annexin-II protein in siIRF-1 THP-1 cells. Myelodysplastic syndrome (MDS) 
      patients with an absence of full-length IRF-1 mRNA also overexpressed Annexin-II. 
      It is plausible that this overexpression may lead to the activation of TLR-4 
      contributing to the increased apoptosis characterizing MDS.
FAU - Maratheftis, Christos I
AU  - Maratheftis CI
AD  - Department of Pathophysiology, School of Medicine, National University of Athens, 
      Athens, Greece.
FAU - Giannouli, Stavroula
AU  - Giannouli S
FAU - Spachidou, Maria P
AU  - Spachidou MP
FAU - Panayotou, George
AU  - Panayotou G
FAU - Voulgarelis, Michael
AU  - Voulgarelis M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Neoplasia
JT  - Neoplasia (New York, N.Y.)
JID - 100886622
RN  - 0 (ANXA2 protein, human)
RN  - 0 (Annexin A2)
RN  - 0 (BAX protein, human)
RN  - 0 (Interferon Regulatory Factor-1)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Amino Acid Sequence
MH  - Annexin A2/*biosynthesis/genetics
MH  - Apoptosis
MH  - Bone Marrow Cells/metabolism/pathology
MH  - Cells, Cultured/drug effects/metabolism
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/biosynthesis/genetics
MH  - Interferon Regulatory Factor-1/*antagonists & inhibitors/genetics/physiology
MH  - Leukemia, Promyelocytic, Acute/metabolism/pathology
MH  - Molecular Sequence Data
MH  - Monocytes/*metabolism
MH  - Myelodysplastic Syndromes/metabolism/pathology
MH  - Protein Structure, Tertiary
MH  - *RNA Interference
MH  - RNA, Messenger/genetics
MH  - RNA, Small Interfering/pharmacology
MH  - Toll-Like Receptor 4/*biosynthesis/genetics
MH  - Transfection
MH  - bcl-2-Associated X Protein/biosynthesis/genetics
PMC - PMC2134898
OTO - NOTNLM
OT  - Annexin II
OT  - IRF-1
OT  - RNAi
OT  - TLR-4
OT  - myelodysplastic syndrome
EDAT- 2007/12/18 09:00
MHDA- 2008/02/26 09:00
PMCR- 2007/12/01
CRDT- 2007/12/18 09:00
PHST- 2007/07/25 00:00 [received]
PHST- 2007/10/08 00:00 [revised]
PHST- 2007/10/09 00:00 [accepted]
PHST- 2007/12/18 09:00 [pubmed]
PHST- 2008/02/26 09:00 [medline]
PHST- 2007/12/18 09:00 [entrez]
PHST- 2007/12/01 00:00 [pmc-release]
AID - 07640 [pii]
AID - 10.1593/neo.07640 [doi]
PST - ppublish
SO  - Neoplasia. 2007 Dec;9(12):1012-20. doi: 10.1593/neo.07640.