PMID- 18086370
OWN - NLM
STAT- MEDLINE
DCOM- 20080314
LR  - 20161124
IS  - 0171-2985 (Print)
IS  - 0171-2985 (Linking)
VI  - 212
IP  - 9-10
DP  - 2007
TI  - Binding of human papilloma virus L1 virus-like particles to dendritic cells is 
      mediated through heparan sulfates and induces immune activation.
PG  - 679-91
AB  - Immunization using human papilloma virus (HPV)-L1 virus-like particles (VLPs) 
      induces a robust and effective immune response, which has recently resulted in 
      the implementation of the HPV-L1 VLP vaccination in health programs. However, 
      during infection, HPV can escape immune surveillance leading to latency and 
      disease. Dendritic cells (DCs) induce effective immune responses after 
      vaccination, but might also induce immune modulation during infection. The 
      interaction of HPV-L1 VLPs with mucosal DCs determines the immune response. 
      However, little is known about the receptors on mucosal DC subsets involved in 
      HPV-L1 VLP binding. Therefore, we set out to investigate the interaction of 
      HPV-L1 VLPs with the different mucosal DC subsets; the subepithelial DCs and 
      Langerhans cells (LCs). We observed strong binding of HPV-L1 VLPs to both DCs and 
      LCs. We did not observe an involvement for C-type lectins such as dendritic 
      cell-specific ICAM-3 grabbing non-integrin (DC-SIGN) and langerin. The HPV-L1 VLP 
      binding to DCs was mediated through heparan sulfates, since it was abrogated by 
      heparinase-II treatment. The heparan sulfate proteoglycan syndecan-3 binds VLPs 
      and is expressed on both DCs and LCs. Binding of VLPs to DCs, but not to LCs, 
      strongly correlated with the levels of heparan sulfates and syndecan-3, 
      suggesting that syndecan-3 is the main receptor for HPV-L1 VLPs on DCs. VLP 
      interaction with DCs resulted in the up-regulation of co-stimulatory molecules 
      and the production of the cytokines IL-6, IL-8, IL-10 and IL-12p40. Our results 
      support an important role for syndecan-3 as a HPV receptor on DCs, which could be 
      important for both vaccine development and understanding HPV pathogenesis.
FAU - de Witte, Lot
AU  - de Witte L
AD  - Department of Molecular Cell Biology and Immunology, VU University Medical 
      Center, van der Boechorststraat 7, 1081 BT, Amsterdam, The Netherlands.
FAU - Zoughlami, Younes
AU  - Zoughlami Y
FAU - Aengeneyndt, Birgit
AU  - Aengeneyndt B
FAU - David, Guido
AU  - David G
FAU - van Kooyk, Yvette
AU  - van Kooyk Y
FAU - Gissmann, Lutz
AU  - Gissmann L
FAU - Geijtenbeek, Teunis B H
AU  - Geijtenbeek TB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071114
PL  - Netherlands
TA  - Immunobiology
JT  - Immunobiology
JID - 8002742
RN  - 0 (Capsid Proteins)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Cytokines)
RN  - 0 (DC-specific ICAM-3 grabbing nonintegrin)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Oncogene Proteins, Viral)
RN  - 0 (Papillomavirus Vaccines)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (SDC3 protein, human)
RN  - 0 (Syndecan-3)
RN  - 6LTE2DNX63 (L1 protein, Human papillomavirus type 16)
RN  - 9050-30-0 (Heparitin Sulfate)
RN  - EC 4.2.2.- (Polysaccharide-Lyases)
RN  - EC 4.2.2.- (heparinase II)
SB  - IM
MH  - Capsid Proteins/*immunology/metabolism
MH  - Cell Adhesion Molecules/metabolism
MH  - Cells, Cultured
MH  - Cytokines/immunology/*metabolism
MH  - Dendritic Cells/cytology/*immunology/metabolism
MH  - Heparitin Sulfate/metabolism
MH  - Human papillomavirus 16/*immunology/metabolism
MH  - Humans
MH  - Langerhans Cells/cytology/*immunology/metabolism
MH  - Lectins, C-Type/metabolism
MH  - Oncogene Proteins, Viral/*immunology/metabolism
MH  - Papillomavirus Vaccines/immunology
MH  - Polysaccharide-Lyases/metabolism
MH  - Receptors, Cell Surface/metabolism
MH  - Syndecan-3/metabolism
MH  - Up-Regulation
EDAT- 2007/12/19 09:00
MHDA- 2008/03/15 09:00
CRDT- 2007/12/19 09:00
PHST- 2007/09/28 00:00 [received]
PHST- 2007/09/28 00:00 [accepted]
PHST- 2007/12/19 09:00 [pubmed]
PHST- 2008/03/15 09:00 [medline]
PHST- 2007/12/19 09:00 [entrez]
AID - S0171-2985(07)00111-8 [pii]
AID - 10.1016/j.imbio.2007.09.006 [doi]
PST - ppublish
SO  - Immunobiology. 2007;212(9-10):679-91. doi: 10.1016/j.imbio.2007.09.006. Epub 2007 
      Nov 14.