PMID- 18087593
OWN - NLM
STAT- MEDLINE
DCOM- 20080201
LR  - 20211020
IS  - 0091-6765 (Print)
IS  - 1552-9924 (Electronic)
IS  - 0091-6765 (Linking)
VI  - 115
IP  - 12
DP  - 2007 Dec
TI  - Maternal-fetal transfer of domoic acid in rats at two gestational time points.
PG  - 1743-6
AB  - BACKGROUND AND OBJECTIVES: Prenatal exposure to asymptomatic doses of domoic acid 
      (DA) causes learning and memory deficits later in life; therefore, we sought to 
      measure distribution of DA in maternal plasma and brain, prenatal brain, and 
      amniotic fluid 1 hr after exposure, a time frame that normally encompasses acute 
      seizure behavior. METHODS: Pregnant rats were given a single intravenous dose of 
      DA (0.6 or 1.6 mg/kg body weight) at either gestational day (GD) 13 or GD20, 
      which correspond to the beginning of rat embryo neurogenesis and the last day of 
      gestation, respectively. Using a direct ELISA, dose-dependent levels of DA were 
      detected in each sample matrix tested. RESULTS: An average of 6.6 and 14 ng DA/g 
      brain tissue was found in GD13 and GD20 prenatal rats, respectively. Brain 
      concentrations of DA in the GD13 prenates were identical to amniotic fluid 
      levels, consistent with no restriction for DA to enter the GD13 prenatal brain. 
      At GD20 the prenatal brain contained half the concentration of DA in the amniotic 
      fluid, and was approximately half that found in the brain of the dams. After 1 
      hr, fetal brain and amniotic fluid contained between 1 and 5% of DA found in the 
      maternal circulation. The amniotic fluid levels of DA in this study were also 
      within the same range measured in stranded California sea lions that showed 
      reproductive failure. CONCLUSIONS: DA crosses the placenta, enters brain tissue 
      of prenates, and accumulates in the amniotic fluid. Amniotic fluid appears to be 
      a useful fluid to monitor DA exposure.
FAU - Maucher, Jennifer M
AU  - Maucher JM
AD  - Marine Biotoxins Program, Center for Coastal Environmental Health and 
      Biomolecular Research, National Oceanic and Atmospheric Administration-National 
      Ocean Service, Charleston, SC 29412, USA.
FAU - Ramsdell, John S
AU  - Ramsdell JS
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Environ Health Perspect
JT  - Environmental health perspectives
JID - 0330411
RN  - M02525818H (domoic acid)
RN  - SIV03811UC (Kainic Acid)
SB  - IM
MH  - Amniotic Fluid/metabolism
MH  - Animals
MH  - Brain/metabolism
MH  - Female
MH  - *Gestational Age
MH  - Kainic Acid/*analogs & derivatives/analysis/blood/metabolism/pharmacokinetics
MH  - *Maternal-Fetal Exchange
MH  - Pregnancy
MH  - Rats
MH  - Tissue Distribution
PMC - PMC2137110
OTO - NOTNLM
OT  - California sea lion
OT  - domoic acid
OT  - prenatal exposure
OT  - rats
EDAT- 2007/12/19 09:00
MHDA- 2008/02/02 09:00
PMCR- 2007/12/01
CRDT- 2007/12/19 09:00
PHST- 2007/05/08 00:00 [received]
PHST- 2007/09/18 00:00 [accepted]
PHST- 2007/12/19 09:00 [pubmed]
PHST- 2008/02/02 09:00 [medline]
PHST- 2007/12/19 09:00 [entrez]
PHST- 2007/12/01 00:00 [pmc-release]
AID - ehp0115-001743 [pii]
AID - 10.1289/ehp.10446 [doi]
PST - ppublish
SO  - Environ Health Perspect. 2007 Dec;115(12):1743-6. doi: 10.1289/ehp.10446.