PMID- 18087663
OWN - NLM
STAT- MEDLINE
DCOM- 20080724
LR  - 20080303
IS  - 0364-216X (Print)
IS  - 0364-216X (Linking)
VI  - 32
IP  - 2
DP  - 2008 Mar
TI  - Transforming growth factor-beta1-antisense modulates the expression of hepatocyte 
      growth factor/scatter factor in keloid fibroblast cell culture.
PG  - 346-52
AB  - Abnormal wound healing processes can result in hypertrophic scars and keloids. 
      Transforming growth factor-beta1 (TGF-beta1) and hepatocyte growth factor/scatter 
      factor (HGF/SF) are biphasic growth factor cytokines in physiologic and 
      pathophysiologic conditions. Findings have shown TGF-beta1 to be pivotal in the 
      formation of keloid tissue. Therefore, neutralizing antibodies may allow wound 
      healing without keloid formation. As reported, TGF-beta1 is antagonized by 
      HGF/SF. Some authors have reported that exogenous administration of HGF/SF 
      prevented scar formation. Hence, this study targeted TGF-beta1 and determined the 
      levels of HGF/SF in fibroblast cell culture. Keloid tissue was taken from seven 
      patients. Another seven patients with mature nonhypertrophic scar served as 
      controls. All tissues were cultured, and fibroblast cultures were used for 
      further experiments. The TGF-beta1 antisense was administered at 3 and 6 
      micromol/ml, and HGF/SF levels were determined after 16, 24, and 48 h of 
      incubation. The levels of HGF/SF showed significant differences after incubation 
      with antisense oligonucleotides. The increasing antisense levels resulted in 
      increased HGF/SF levels (up to 87.66 pg/ml after 48 h of incubation). In 
      conclusion, targeting TGF-beta1 resulted in significantly increased levels of 
      HGF/SF. The clinical relevance could include the use of locally administered 
      HGF/SF in protein or gene form to minimize formation of keloids. Nevertheless, 
      wound healing is the result of many interacting cytokines, so neutralizing or 
      targeting one protein could result in no significant effect.
FAU - Naim, R
AU  - Naim R
AD  - Department of Otolaryngology, Head and Neck Surgery, University of Saarland, 
      Homburg/Saar, Kirrberger Strasse, 66421 Homburg, Germany. ramin.naim@gmail.com
FAU - Naumann, A
AU  - Naumann A
FAU - Barnes, J
AU  - Barnes J
FAU - Sauter, A
AU  - Sauter A
FAU - Hormann, K
AU  - Hormann K
FAU - Merkel, D
AU  - Merkel D
FAU - Aust, W
AU  - Aust W
FAU - Braun, T
AU  - Braun T
FAU - Bloching, M
AU  - Bloching M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Aesthetic Plast Surg
JT  - Aesthetic plastic surgery
JID - 7701756
RN  - 0 (DNA, Antisense)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Adult
MH  - Blotting, Western
MH  - Cicatrix/*metabolism
MH  - DNA, Antisense/*genetics
MH  - Female
MH  - Fibroblasts/*cytology/*metabolism
MH  - Hepatocyte Growth Factor/*metabolism
MH  - Humans
MH  - Keloid/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Transforming Growth Factor beta1/*genetics/*metabolism
MH  - *Up-Regulation
MH  - Wound Healing/*physiology
EDAT- 2007/12/19 09:00
MHDA- 2008/07/25 09:00
CRDT- 2007/12/19 09:00
PHST- 2007/12/19 09:00 [pubmed]
PHST- 2008/07/25 09:00 [medline]
PHST- 2007/12/19 09:00 [entrez]
AID - 10.1007/s00266-007-9078-6 [doi]
PST - ppublish
SO  - Aesthetic Plast Surg. 2008 Mar;32(2):346-52. doi: 10.1007/s00266-007-9078-6.