PMID- 18087893
OWN - NLM
STAT- MEDLINE
DCOM- 20080131
LR  - 20191110
IS  - 0856-6496 (Print)
IS  - 0856-6496 (Linking)
VI  - 9
IP  - 3
DP  - 2007 Sep
TI  - The quality of sputum smear microscopy diagnosis of pulmonary tuberculosis in Dar 
      es Salaam, Tanzania.
PG  - 164-8
AB  - This study was carried out to determine the rate of agreement or disagreement of 
      microscopy reading and culture positivity rate among smear positixe and negative 
      specimens between peripheral tuberculosis diagnostic centres (PDCs) and Central 
      Reference luberculosis laboratory (CTRL). In this study 13 PDCs in Dar es Salaam, 
      Tanzania were involved. Lot Quality Assurance Sampling (LQAS) method was used to 
      collect 222 sputum smear slides. A total of 190 morning sputum specimens with 
      corresponding slides were selected for culture. First readings were done by 
      technicians at PDCs and thereafter selected slides and specimens were sent to 
      CTRL for re-examination and culture. Culture results were used as a gold 
      standard. Of 222 slides selected, 214 were suitable for re-examination. 
      Percentage of agreement of smear reading between PDCs and CTRL was 42.9% and 100% 
      for positive and negative slides, respectively. Measure of agreement (Kappa 
      statistic) was 0.5, indicating moderate agreement. Of 190 samples cultured, 
      percentage of agreement between smear reading from PDCs and CTRL was 37% and 
      88.9% for smear positive and negative slides, respectively. Kappa statistic was 
      0.3 indicating poor-fair agreements. Comparison of smear reading from PDCs with 
      culture showed sensitivity of 36.9% and specificity of 88.9%. Comparison of smear 
      readings from CTRL with culture results showed sensitivity of 95.6% and 
      specificity of 98.6%. In conclusion there was inadequate performance in diagnosis 
      of TB using smear microscopy among peripheral diagnostic centres in Dar es 
      Salaam. This calls for immediate and rigorous measures to improve the quality of 
      smear microscopy. It is therefore important to strengthen the capacity of 
      laboratory personnel in smear microscopy techniques through supportive 
      supervision and training.
FAU - Mfinanga, G S
AU  - Mfinanga GS
AD  - National Institute for Medical Research, Muhimbili Medical Research Centre, P.O. 
      Box 3436, Dar es Salaam, Tanzania. gsmfinanga@yahoo.com
FAU - Ngadaya, E
AU  - Ngadaya E
FAU - Mtandu, R
AU  - Mtandu R
FAU - Mutayoba, B
AU  - Mutayoba B
FAU - Basra, D
AU  - Basra D
FAU - Kimaro, G
AU  - Kimaro G
FAU - Chonde, T M
AU  - Chonde TM
FAU - Ngowi, P
AU  - Ngowi P
FAU - Mfaume, S
AU  - Mfaume S
FAU - Kilale, A M
AU  - Kilale AM
FAU - Egwaga, S
AU  - Egwaga S
FAU - Kitua, A Y
AU  - Kitua AY
LA  - eng
PT  - Journal Article
PT  - Multicenter Study
PT  - Research Support, Non-U.S. Gov't
PL  - Tanzania
TA  - Tanzan Health Res Bull
JT  - Tanzania health research bulletin
JID - 101262014
SB  - IM
MH  - Clinical Laboratory Techniques/*statistics & numerical data
MH  - Cross-Sectional Studies
MH  - Humans
MH  - *Mycobacterium tuberculosis
MH  - *Quality Assurance, Health Care
MH  - Reproducibility of Results
MH  - Sampling Studies
MH  - Sputum/*microbiology
MH  - Tanzania
MH  - Tuberculosis, Pulmonary/*diagnosis
EDAT- 2007/12/20 09:00
MHDA- 2008/02/01 09:00
CRDT- 2007/12/20 09:00
PHST- 2007/12/20 09:00 [pubmed]
PHST- 2008/02/01 09:00 [medline]
PHST- 2007/12/20 09:00 [entrez]
AID - 10.4314/thrb.v9i3.14323 [doi]
PST - ppublish
SO  - Tanzan Health Res Bull. 2007 Sep;9(3):164-8. doi: 10.4314/thrb.v9i3.14323.