PMID- 18088182
OWN - NLM
STAT- MEDLINE
DCOM- 20080206
LR  - 20071219
IS  - 0033-7587 (Print)
IS  - 0033-7587 (Linking)
VI  - 168
IP  - 6
DP  - 2007 Dec
TI  - The characterization and transmissibility of chromosome aberrations induced in 
      peripheral blood lymphocytes by in vitro alpha-particle radiation.
PG  - 666-74
AB  - Peripheral blood lymphocytes were irradiated in vitro with (213)Bi alpha 
      particles at doses of 0, 10, 20, 50, 100, 200 and 500 mGy. Chromosome analysis 
      was performed on 47-h cultures using single-color fluorescence in situ 
      hybridization (FISH) to paint chromosomes 1, 3 and 5. The whole genome was 
      analyzed for unstable aberrations to derive aberration frequencies and determine 
      cell stability. The dose response for dicentrics was 33.60 +/- 0.47 x 10(-2) per 
      Gy. A more detailed analysis revealed that the majority of aberrations scored as 
      dicentrics were part of complex/multiple aberrations, with the proportion of 
      cells containing complexes increasing with dose. Cells containing aberrations 
      involving painted chromosomes (FISH aberrations) were further classified 
      according to cell stability and complexity. The majority of cells with FISH 
      aberrations were unstable. The proportion of aberrant FISH cells with 
      complex/multiple aberrations ranged from 56% at 10 mGy to 89% at 500 mGy. A 
      linear dose response for genomic frequencies of translocations in stable cells 
      fitted the data from 0 to 200 mGy with a dose response of 7.90 +/- 0.98 x 10(-2) 
      per Gy, thus indicating that they are likely to be observed in peripheral blood 
      lymphocytes from individuals with past or chronic exposure to high-LET radiation. 
      Comparisons with the dose response for low-LET radiation suggest an RBE of 13.6 
      for dicentrics in all cells and 3.2 for translocations in stable cells. Since 
      stochastic effects of radiation are attributable to genetic changes in viable 
      cells, translocations in stable cells may be a better measure when considering 
      the comparative risks of different qualities of radiation.
FAU - Tawn, E Janet
AU  - Tawn EJ
AD  - Westlakes Research Institute, Moor Row, Cumbria, United Kingdom. 
      ejtawn@uclan.ac.uk
FAU - Whitehouse, Caroline A
AU  - Whitehouse CA
FAU - De Ruyck, Kim
AU  - De Ruyck K
FAU - Hodgson, Leanne
AU  - Hodgson L
FAU - Vandenbulcke, Katia
AU  - Vandenbulcke K
FAU - Thierens, Hubert
AU  - Thierens H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Radiat Res
JT  - Radiation research
JID - 0401245
SB  - IM
MH  - *Alpha Particles
MH  - Cells, Cultured
MH  - Chromosome Aberrations/*radiation effects
MH  - Genome, Human/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphocytes/*metabolism/*radiation effects
EDAT- 2007/12/20 09:00
MHDA- 2008/02/07 09:00
CRDT- 2007/12/20 09:00
PHST- 2007/01/23 00:00 [received]
PHST- 2007/07/19 00:00 [accepted]
PHST- 2007/12/20 09:00 [pubmed]
PHST- 2008/02/07 09:00 [medline]
PHST- 2007/12/20 09:00 [entrez]
AID - RR0969 [pii]
AID - 10.1667/RR0969.1 [doi]
PST - ppublish
SO  - Radiat Res. 2007 Dec;168(6):666-74. doi: 10.1667/RR0969.1.