PMID- 18089344
OWN - NLM
STAT- MEDLINE
DCOM- 20080220
LR  - 20131121
IS  - 0041-1345 (Print)
IS  - 0041-1345 (Linking)
VI  - 39
IP  - 10
DP  - 2007 Dec
TI  - Effect of folate, vitamin B6, and vitamin B12 intake and MTHFR C677T polymorphism 
      on homocysteine concentrations of renal transplant recipients.
PG  - 3163-5
AB  - Plasma hyperhomocysteinemia (HHcy) is considered a risk factor for chronic 
      allograft dysfunction (CAD), the main cause of functional loss in transplant 
      recipients. Genetic polymorphisms that alter enzymes involved in homocysteine 
      (Hcy) metabolism, such as methylenetetrahydrofolate reductase (MTHFR), and 
      vitamin deficiency can result in HHcy. The objectives of this study were to 
      investigate the relationship between HHcy and CAD development, and to evaluate 
      the effect of intake of folate and vitamins B6 and B12 as well as MTHFR C677T 
      polymorphism on Hcy concentrations. Ninety-eight renal transplant recipients 
      including 48 showing CAD and 50 with normal renal function (NRF), were included 
      in this cross-sectional study. Peripheral blood samples were collected for plasma 
      Hcy quantification by liquid chromatography/sequential mass spectrometry 
      (LC-MS/MS), and for MTHFR polymorphism analysis using polymerase chain 
      reaction-restriction fragment length polymorphism. Dietary intake was evaluated 
      using a nutritional questionnaire. HHcy (P=.002) and higher mean concentrations 
      of Hcy (P=.029) were associated with CAD. An association was observed between 
      HHcy and 677T variant allele in the CAD group (P=.0005). There was no correlation 
      between Hcy concentration and folate, vitamin B6 or vitamin B12 intake in the CAD 
      group. However, a negative correlation was observed between Hcy concentration and 
      folate intake (P=.043), and also between Hcy concentration and vitamin B6 intake 
      (P=.030) in the NRF group. According to our study, HHcy is associated with CAD 
      development. In patients with CAD, MTHFR polymorphism seems to have a greater 
      effect on the Hcy concentration than the vitamin intake. Increased folate and 
      vitamin B6 intakes seem to reduce Hcy concentrations among transplant recipients 
      with NRF, and could contribute to reducing the risk of CAD development.
FAU - Biselli, P M
AU  - Biselli PM
AD  - Research Unit in Genetics and Molecular Biology (UPGEM) Sao Jose do Rio Preto 
      Medical School (FAMERP), SP, Brazil.
FAU - Sanches de Alvarenga, M P
AU  - Sanches de Alvarenga MP
FAU - Abbud-Filho, M
AU  - Abbud-Filho M
FAU - Ferreira-Baptista, M A S
AU  - Ferreira-Baptista MA
FAU - Galbiatti, A L S
AU  - Galbiatti AL
FAU - Goto, M T Y
AU  - Goto MT
FAU - Cardoso, M A
AU  - Cardoso MA
FAU - Eberlin, M N
AU  - Eberlin MN
FAU - Haddad, R
AU  - Haddad R
FAU - Goloni-Bertollo, E M
AU  - Goloni-Bertollo EM
FAU - Pavarino-Bertelli, E C
AU  - Pavarino-Bertelli EC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Transplant Proc
JT  - Transplantation proceedings
JID - 0243532
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - 8059-24-3 (Vitamin B 6)
RN  - 935E97BOY8 (Folic Acid)
RN  - EC 1.5.1.20 (Methylenetetrahydrofolate Reductase (NADPH2))
RN  - P6YC3EG204 (Vitamin B 12)
SB  - IM
MH  - Cross-Sectional Studies
MH  - Folic Acid/*therapeutic use
MH  - Homocysteine/*blood
MH  - Humans
MH  - Hyperhomocysteinemia/prevention & control
MH  - Kidney Function Tests
MH  - Kidney Transplantation/adverse effects/*physiology
MH  - Methylenetetrahydrofolate Reductase (NADPH2)/*genetics
MH  - *Polymorphism, Single Nucleotide
MH  - Postoperative Complications/prevention & control
MH  - Vitamin B 12/*therapeutic use
MH  - Vitamin B 6/*therapeutic use
EDAT- 2007/12/20 09:00
MHDA- 2008/02/21 09:00
CRDT- 2007/12/20 09:00
PHST- 2006/12/03 00:00 [received]
PHST- 2007/06/15 00:00 [revised]
PHST- 2007/08/08 00:00 [accepted]
PHST- 2007/12/20 09:00 [pubmed]
PHST- 2008/02/21 09:00 [medline]
PHST- 2007/12/20 09:00 [entrez]
AID - S0041-1345(07)01126-8 [pii]
AID - 10.1016/j.transproceed.2007.08.098 [doi]
PST - ppublish
SO  - Transplant Proc. 2007 Dec;39(10):3163-5. doi: 10.1016/j.transproceed.2007.08.098.