PMID- 18089402
OWN - NLM
STAT- MEDLINE
DCOM- 20080220
LR  - 20181201
IS  - 0041-1345 (Print)
IS  - 0041-1345 (Linking)
VI  - 39
IP  - 10
DP  - 2007 Dec
TI  - Fibronectin suppresses lipopolysaccharide-induced liver damage and promotes the 
      cytoprotection abilities of hepatocyte-like cells derived from human bone marrow 
      mesenchymal stem cells.
PG  - 3444-5
AB  - We isolated mesenchymal stem cells (MSCs) from adult human bone marrow. By using 
      reverse-transcription polymerase chain reactions, we confirmed that MSCs 
      possessed the potential to differentiate into hepatocyte-like cells (MSC-HLCs) 
      with the expression of hepatocyte-specific marker genes. We further observed that 
      fibronectin (FN) treatment significantly inhibited lipopolysaccharide 
      (LPS)-induced apoptotic activities in FN-treated MSC-HLCs, as detected by caspase 
      3 enzyme-linked immunosorbent (ELISA) and terminal dUTP nick-end labeling (TUNEL) 
      assays (P<.05). The FN-treated MSC-HLCs were transplanted into SCID mice with or 
      without LPS injection. This study demonstrated that FN treatment improved liver 
      function repair and survival rates among LPS-treated SCID mice.
FAU - Kao, C-L
AU  - Kao CL
AD  - Department of Physical Medicine and Rehabilitation, Taipei Veterans General 
      Hospital, and Institute of Clinical Medicine, National Yang-Ming University, 
      Taipei, Taiwan.
FAU - Lin, H-T
AU  - Lin HT
FAU - Chen, Y-W
AU  - Chen YW
FAU - Chen, Y-C
AU  - Chen YC
FAU - Tsai, F-T
AU  - Tsai FT
FAU - Chang, Y-L
AU  - Chang YL
FAU - Chiou, S-H
AU  - Chiou SH
FAU - Sheu, D-C
AU  - Sheu DC
FAU - Ku, H-H
AU  - Ku HH
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Transplant Proc
JT  - Transplantation proceedings
JID - 0243532
RN  - 0 (Fibronectins)
RN  - 0 (Lipopolysaccharides)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Caspase 3/analysis
MH  - Cell Differentiation
MH  - Cell Survival/drug effects
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Fibronectins/*pharmacology
MH  - Hepatocytes/*cytology
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Lipopolysaccharides/*antagonists & inhibitors/*pharmacology
MH  - Liver Function Tests
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - Mesenchymal Stem Cells/*cytology
MH  - Mice
MH  - Mice, SCID
MH  - Transplantation, Heterologous
EDAT- 2007/12/20 09:00
MHDA- 2008/02/21 09:00
CRDT- 2007/12/20 09:00
PHST- 2006/09/28 00:00 [received]
PHST- 2007/02/05 00:00 [revised]
PHST- 2007/03/05 00:00 [accepted]
PHST- 2007/12/20 09:00 [pubmed]
PHST- 2008/02/21 09:00 [medline]
PHST- 2007/12/20 09:00 [entrez]
AID - S0041-1345(07)00963-3 [pii]
AID - 10.1016/j.transproceed.2007.03.104 [doi]
PST - ppublish
SO  - Transplant Proc. 2007 Dec;39(10):3444-5. doi: 10.1016/j.transproceed.2007.03.104.