PMID- 18092862
OWN - NLM
STAT- MEDLINE
DCOM- 20080306
LR  - 20161124
IS  - 0730-7268 (Print)
IS  - 0730-7268 (Linking)
VI  - 27
IP  - 1
DP  - 2008 Jan
TI  - Oxidative damage in unfertilized eggs of Chinese rare minnow (Gobiocypris rarus) 
      exposed to nonylphenol.
PG  - 213-9
AB  - In the present study, female Chinese rare minnows (Gobiocypris rarus) were used 
      as in vivo models and exposed to nonylphenol (NP) at concentrations of 1 to 200 
      microg/L for 21 d under semistatic conditions. Molecular biomarkers of oxidative 
      stress were measured in unfertilized eggs and included reactive oxygen species 
      (ROS), lipid peroxidation products (thiobarbituric acid- reactive substances 
      [TBARS] and protein carbonyl), superoxide dismutase activity, and glutathione. 
      Cathepsin D activity as an indicator of egg viability also was assayed. 
      Nonylphenol induced ROS formation in unfertilized eggs in all exposed groups 
      compared to the controls. The levels of protein carbonyl and TBARS in 
      unfertilized eggs were significantly increased (p < 0.05) at 10 to 200 and 100 to 
      200 microg/L, respectively. Good positive correlations were shown between ROS 
      induction and levels of TBARS and protein carbonyl in eggs (R = 0.918, p < 0.05 
      and R = 0.784, p < 0.05, respectively). Superoxide dismutase activity in eggs was 
      significantly inhibited (p < 0.05) in the 50 to 200 microg/L exposure groups. 
      Glutathione levels in eggs were significantly depleted (p < 0.05) at 100 to 200 
      microg/L concentrations. In addition, ROS induction resulted in oxidative damage 
      to lipid and protein in chorions. Significant reductions (p < 0.05) of the 
      protein and lipid contents in chorions were both found in the 50 to 200 microg/L 
      exposure groups. A previous study found that NP exposure could lead to chorion 
      thinning in zebra fish. Thus, the reductions in protein and lipid contents in 
      chorion could be the reason for chorion thinning by NP exposure. Meanwhile, 
      cathepsin D activity was significantly inhibited (p < 0.05) in all exposure 
      groups. The results demonstrated that NP-induced oxidative stress could damage 
      the chorion of unfertilized eggs and lead to a decline in gamete quality in 
      female Chinese rare minnow.
FAU - Zhang, Xian
AU  - Zhang X
AD  - State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of 
      Hydrobiology, Chinese Academy of Sciences, Wuhan, People's Republic of China.
FAU - Yang, Fangxing
AU  - Yang F
FAU - Cai, Ya Q
AU  - Cai YQ
FAU - Xu, Ying
AU  - Xu Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Environ Toxicol Chem
JT  - Environmental toxicology and chemistry
JID - 8308958
RN  - 0 (Biomarkers)
RN  - 0 (Environmental Pollutants)
RN  - 0 (Lipids)
RN  - 0 (Phenols)
RN  - 0 (Proteins)
RN  - 0 (Reactive Oxygen Species)
RN  - 79F6A2ILP5 (nonylphenol)
SB  - IM
MH  - Animals
MH  - Biomarkers/analysis
MH  - Cell Survival
MH  - Chorion/drug effects
MH  - *Cyprinidae
MH  - Environmental Pollutants/adverse effects
MH  - Female
MH  - Lipids/analysis
MH  - Ovum/drug effects/metabolism/pathology
MH  - Oxidative Stress/*drug effects
MH  - Phenols/*adverse effects
MH  - Proteins/analysis
MH  - Reactive Oxygen Species/metabolism
EDAT- 2007/12/21 09:00
MHDA- 2008/03/07 09:00
CRDT- 2007/12/21 09:00
PHST- 2007/01/31 00:00 [received]
PHST- 2007/05/18 00:00 [accepted]
PHST- 2007/12/21 09:00 [pubmed]
PHST- 2008/03/07 09:00 [medline]
PHST- 2007/12/21 09:00 [entrez]
AID - 07-074 [pii]
AID - 10.1897/07-074.1 [doi]
PST - ppublish
SO  - Environ Toxicol Chem. 2008 Jan;27(1):213-9. doi: 10.1897/07-074.1.