PMID- 18093416
OWN - NLM
STAT- MEDLINE
DCOM- 20080131
LR  - 20191210
IS  - 0146-4760 (Print)
IS  - 0146-4760 (Linking)
VI  - 31
IP  - 9
DP  - 2007 Nov-Dec
TI  - Determination of illicit drugs and their metabolites in human urine by liquid 
      chromatography tandem mass spectrometry including relative ion intensity 
      criterion.
PG  - 573-80
AB  - A method, using 0.5 mL of urine, was developed for the simultaneous determination 
      of ecgonine methyl ester, benzoylecgonine, morphine, codeine, 6-acetylmorphine, 
      amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine (MDA), 
      3,4-methylenedioxymethamphetamine (MDMA), methadone, 
      2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP), and d-lysergic acid 
      diethylamide (LSD). The analysis was performed by liquid chromatography with 
      tandem mass spectrometry, after solid-phase extraction in the presence of their 
      deuterated analogues. Reversed-phase separation on an Atlantis dC18 column was 
      achieved in 12.5 min, under gradient conditions. The method was fully validated, 
      including linearity (1-2000 microg/L for ecgonine methyl ester, benzoylecgonine, 
      6-acetylmorphine, methamphetamine, MDMA, and EDDP; 2-2000 microg/L for morphine, 
      codeine, MDA, and methadone; 2-1000 microg/L for amphetamine, and 0.2-100 
      microg/L for LSD; r(2)>0.99); recovery (>65%), within-day and between-day 
      precision, and accuracy (CV and MRE<15%); limit of detection (0.1 microg/L for 
      LSD, 0.5 microg/L for ecgonine methyl ester, benzoylecgonine, methamphetamine, 
      MDMA, 6-monoacetylmorphine, and EDDP, and 1 microg/L for amphetamine, MDA, 
      morphine, and methadone); quantitation (lowest level of the calibration curve); 
      relative ion intensities, freeze-and-thaw stability, and matrix effect. The 
      procedure showed to be sensitive and specific, and was applied to real cases and 
      quality control samples from a quality control program.
FAU - Concheiro, Marta
AU  - Concheiro M
AD  - University of Santiago de Compostela, Forensic Toxicology Service, Spain.
FAU - De Castro, Ana
AU  - De Castro A
FAU - Quintela, Oscar
AU  - Quintela O
FAU - Cruz, Angelines
AU  - Cruz A
FAU - Lopez-Rivadulla, Manuel
AU  - Lopez-Rivadulla M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
PL  - England
TA  - J Anal Toxicol
JT  - Journal of analytical toxicology
JID - 7705085
RN  - 0 (Amphetamines)
RN  - 0 (Illicit Drugs)
RN  - 0 (Pyrrolidines)
RN  - 5353I8I6YS (benzoylecgonine)
RN  - 76I7G6D29C (Morphine)
RN  - 8NA5SWF92O (Lysergic Acid Diethylamide)
RN  - I5Y540LHVR (Cocaine)
RN  - UC6VBE7V1Z (Methadone)
RN  - Y35FJB3QBJ (ecgonine methyl ester)
RN  - Z3LC48U94I (2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine)
SB  - IM
MH  - Amphetamines/urine
MH  - Chromatography, Liquid
MH  - Cocaine/analogs & derivatives/urine
MH  - Humans
MH  - Illicit Drugs/*urine
MH  - Lysergic Acid Diethylamide/urine
MH  - Methadone/urine
MH  - Morphine/urine
MH  - Pyrrolidines/urine
MH  - Tandem Mass Spectrometry
EDAT- 2007/12/21 09:00
MHDA- 2008/02/01 09:00
CRDT- 2007/12/21 09:00
PHST- 2007/12/21 09:00 [pubmed]
PHST- 2008/02/01 09:00 [medline]
PHST- 2007/12/21 09:00 [entrez]
AID - 10.1093/jat/31.9.573 [doi]
PST - ppublish
SO  - J Anal Toxicol. 2007 Nov-Dec;31(9):573-80. doi: 10.1093/jat/31.9.573.