PMID- 18093585
OWN - NLM
STAT- MEDLINE
DCOM- 20080416
LR  - 20131121
IS  - 0014-4835 (Print)
IS  - 0014-4835 (Linking)
VI  - 86
IP  - 2
DP  - 2008 Feb
TI  - Expression of brain-derived neurotrophic factor in cholinergic and dopaminergic 
      amacrine cells in the rat retina and the effects of constant light rearing.
PG  - 335-43
AB  - Brain-derived neurotrophic factor (BDNF) regulates many aspects of neuronal 
      development, including survival, axonal and dendritic growth and synapse 
      formation. Despite recent advances in our understanding of the functional 
      significance of BDNF in retinal development, the retinal cell types expressing 
      BDNF remains poorly defined. The goal of the present study was to determine the 
      localization of BDNF in the mammalian retina, with special focus on the subtypes 
      of amacrine cells, and to characterize, at the cellular level, the effects of 
      constant light exposure during early postnatal period on retinal expression of 
      BDNF. Retinas from 3-week-old rats reared in a normal light cycle or constant 
      light were subjected to double immunofluorescence staining using antibodies to 
      BDNF and retinal cell markers. BDNF immunoreactivity was localized to ganglion 
      cells, cholinergic amacrine cells and dopaminergic amacrine cells, but not to AII 
      amacrine cells regardless of rearing conditions. Approximately 75% of 
      BDNF-positive cells in the inner nuclear layer were cholinergic amacrine cells in 
      animals reared in a normal lighting condition. While BDNF immunoreactivity in 
      ganglion cells and cholinergic amacrine cells was significantly increased by 
      constant light rearing, which in dopaminergic amacrine cells was apparently 
      unaltered. The overall structure of the retina and the density of ganglion cells, 
      cholinergic amacrine cells and AII amacrine cells were unaffected by rearing 
      conditions, whereas the density of dopaminergic amacrine cells was significantly 
      increased by constant light rearing. The present results indicate that 
      cholinergic amacrine cells are the primary source of BDNF in the inner nuclear 
      layer of the rat retina and provide the first evidence that cholinergic amacrine 
      cells may be involved in the visual activity-dependent regulation of retinal 
      development through the production of BDNF. The present data also suggest that 
      the production or survival of dopaminergic amacrine cells is regulated by early 
      visual experience.
FAU - Fujieda, Hiroki
AU  - Fujieda H
AD  - Department of Anatomy, School of Medicine, Tokyo Women's Medical University, 
      Tokyo, Japan. hfujieda@research.twmu.ac.jp
FAU - Sasaki, Hiroshi
AU  - Sasaki H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071117
PL  - England
TA  - Exp Eye Res
JT  - Experimental eye research
JID - 0370707
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - N9YNS0M02X (Acetylcholine)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Acetylcholine/biosynthesis
MH  - Amacrine Cells/*metabolism/radiation effects
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Dopamine/biosynthesis
MH  - Female
MH  - Light
MH  - Male
MH  - Rats
MH  - Rats, Wistar
MH  - Retina/*metabolism/radiation effects
MH  - Retinal Ganglion Cells/metabolism/radiation effects
EDAT- 2007/12/21 09:00
MHDA- 2008/04/17 09:00
CRDT- 2007/12/21 09:00
PHST- 2007/07/07 00:00 [received]
PHST- 2007/10/26 00:00 [revised]
PHST- 2007/11/08 00:00 [accepted]
PHST- 2007/12/21 09:00 [pubmed]
PHST- 2008/04/17 09:00 [medline]
PHST- 2007/12/21 09:00 [entrez]
AID - S0014-4835(07)00321-1 [pii]
AID - 10.1016/j.exer.2007.11.005 [doi]
PST - ppublish
SO  - Exp Eye Res. 2008 Feb;86(2):335-43. doi: 10.1016/j.exer.2007.11.005. Epub 2007 
      Nov 17.