PMID- 18094232
OWN - NLM
STAT- MEDLINE
DCOM- 20080114
LR  - 20240419
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 27
IP  - 51
DP  - 2007 Dec 19
TI  - Two distinct heterotypic channels mediate gap junction coupling between astrocyte 
      and oligodendrocyte connexins.
PG  - 13949-57
AB  - Genetic diseases demonstrate that the normal function of CNS myelin depends on 
      connexin32 (Cx32) and Cx47, gap junction (GJ) proteins expressed by 
      oligodendrocytes. GJs couple oligodendrocytes and astrocytes (O/A channels) as 
      well as astrocytes themselves (A/A channels). Because astrocytes express 
      different connexins (Cx30 and Cx43), O/A channels must be heterotypic, whereas 
      A/A channels may be homotypic or heterotypic. Using electrophysiological and 
      immunocytochemical approaches, we found that Cx47/Cx43 and Cx32/Cx30 efficiently 
      formed functional channels, but other potential heterotypic O/A and A/A pairs did 
      not. These results suggest that Cx30/Cx30 and Cx43/Cx43 channels mediate A/A 
      coupling, and Cx47/Cx43 and Cx32/Cx30 channels mediate O/A coupling. Furthermore, 
      Cx47/Cx43 and Cx32/Cx30 channels have distinct macroscopic and single-channel 
      properties and different dye permeabilities. Finally, Cx47 mutants that cause 
      Pelizaeus-Merzbacher-like disease do not efficiently form functional channels 
      with Cx43, indicating that disrupted Cx47/Cx43 channels cause this disease.
FAU - Orthmann-Murphy, Jennifer L
AU  - Orthmann-Murphy JL
AD  - Department of Neurology, University of Pennsylvania School of Medicine, 
      Philadelphia, Pennsylvania 19104-6077, USA. lorthman@mail.med.upenn.edu
FAU - Freidin, Mona
AU  - Freidin M
FAU - Fischer, Esther
AU  - Fischer E
FAU - Scherer, Steven S
AU  - Scherer SS
FAU - Abrams, Charles K
AU  - Abrams CK
LA  - eng
GR  - R01 NS050705-03/NS/NINDS NIH HHS/United States
GR  - R01 NS055284/NS/NINDS NIH HHS/United States
GR  - K02 NS050345-03/NS/NINDS NIH HHS/United States
GR  - NS054363/NS/NINDS NIH HHS/United States
GR  - NS043560/NS/NINDS NIH HHS/United States
GR  - NS50345/NS/NINDS NIH HHS/United States
GR  - K02 NS050345-02/NS/NINDS NIH HHS/United States
GR  - NS55284/NS/NINDS NIH HHS/United States
GR  - R01 NS050705-02/NS/NINDS NIH HHS/United States
GR  - F30 NS054363/NS/NINDS NIH HHS/United States
GR  - R01 NS050705/NS/NINDS NIH HHS/United States
GR  - K02 NS050345/NS/NINDS NIH HHS/United States
GR  - R01 NS043560/NS/NINDS NIH HHS/United States
GR  - NS050705/NS/NINDS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Connexins)
RN  - 0 (Ion Channels)
SB  - IM
MH  - Astrocytes/metabolism/*physiology
MH  - Connexins/chemistry/*physiology
MH  - Gap Junctions/chemistry/*metabolism/physiology
MH  - HeLa Cells
MH  - Humans
MH  - Ion Channel Gating/physiology
MH  - Ion Channels/chemistry/*physiology
MH  - Oligodendroglia/metabolism/*physiology
PMC - PMC6673504
EDAT- 2007/12/21 09:00
MHDA- 2008/01/15 09:00
PMCR- 2008/06/19
CRDT- 2007/12/21 09:00
PHST- 2007/12/21 09:00 [pubmed]
PHST- 2008/01/15 09:00 [medline]
PHST- 2007/12/21 09:00 [entrez]
PHST- 2008/06/19 00:00 [pmc-release]
AID - 27/51/13949 [pii]
AID - 3294191 [pii]
AID - 10.1523/JNEUROSCI.3395-07.2007 [doi]
PST - ppublish
SO  - J Neurosci. 2007 Dec 19;27(51):13949-57. doi: 10.1523/JNEUROSCI.3395-07.2007.