PMID- 18094241
OWN - NLM
STAT- MEDLINE
DCOM- 20080114
LR  - 20211020
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 27
IP  - 51
DP  - 2007 Dec 19
TI  - Reciprocal regulation of presynaptic and postsynaptic proteins in bipolar spiral 
      ganglion neurons by neurotrophins.
PG  - 14023-34
AB  - A unifying principle of sensory system organization is feature extraction by 
      modality-specific neuronal maps in which arrays of neurons show systematically 
      varied response properties and receptive fields. Only beginning to be understood, 
      however, are the mechanisms by which these graded systems are established. In the 
      peripheral auditory system, we have shown previously that the intrinsic firing 
      features of spiral ganglion neurons are influenced by brain-derived neurotrophic 
      factor (BDNF) and neurotrophin-3 (NT-3). We now show that is but a part of a 
      coordinated package of neurotrophin actions that also includes effects on 
      presynaptic and postsynaptic proteins, thus encompassing the input, transmission, 
      and output functions of the spiral ganglion neurons. Using immunocytochemical 
      methods, we determined that proteins targeted to opposite ends of the neuron were 
      organized and regulated in a reciprocal manner. AMPA receptor subunits GluR2 and 
      GluR3 were enriched in base neurons compared with their apex counterparts. This 
      distribution pattern was enhanced by exposure to BDNF but reduced by NT-3. 
      SNAP-25 and synaptophysin were distributed and regulated in the mirror image: 
      enriched in the apex, enhanced by NT-3 and reduced by BDNF. Moreover, we used a 
      novel coculture to identify potential endogenous sources of neurotrophins by 
      showing that sensory receptors from different cochlear regions were capable of 
      altering presynaptic and postsynaptic protein levels in these neurons. From these 
      studies, we suggest that BDNF and NT-3, which are systematically distributed in 
      complementary gradients, are responsible for orchestrating a comprehensive set of 
      electrophysiological specializations along the frequency contour of the cochlea.
FAU - Flores-Otero, Jacqueline
AU  - Flores-Otero J
AD  - Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, New 
      Jersey 08854, USA.
FAU - Xue, Hui Zhong
AU  - Xue HZ
FAU - Davis, Robin L
AU  - Davis RL
LA  - eng
GR  - R01 DC01856/DC/NIDCD NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Membrane Proteins)
RN  - 0 (Nerve Growth Factors)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Cells, Cultured
MH  - Membrane Proteins/*physiology/ultrastructure
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Nerve Growth Factors/*physiology/ultrastructure
MH  - Presynaptic Terminals/chemistry/*metabolism/physiology/ultrastructure
MH  - Spiral Ganglion/cytology/*metabolism/physiology
PMC - PMC6673512
EDAT- 2007/12/21 09:00
MHDA- 2008/01/15 09:00
PMCR- 2008/06/19
CRDT- 2007/12/21 09:00
PHST- 2007/12/21 09:00 [pubmed]
PHST- 2008/01/15 09:00 [medline]
PHST- 2007/12/21 09:00 [entrez]
PHST- 2008/06/19 00:00 [pmc-release]
AID - 27/51/14023 [pii]
AID - 3299909 [pii]
AID - 10.1523/JNEUROSCI.3219-07.2007 [doi]
PST - ppublish
SO  - J Neurosci. 2007 Dec 19;27(51):14023-34. doi: 10.1523/JNEUROSCI.3219-07.2007.