PMID- 18094328
OWN - NLM
STAT- MEDLINE
DCOM- 20080425
LR  - 20211027
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 111
IP  - 6
DP  - 2008 Mar 15
TI  - The inhibitory receptor LILRB1 modulates the differentiation and regulatory 
      potential of human dendritic cells.
PG  - 3090-6
AB  - Dendritic cells (DCs) link innate and adaptive immunity, initiating and 
      regulating effector cell responses. They ubiquitously express members of the LILR 
      (ILT, LIR, CD85) family of molecules, some of which recognize self-HLA molecules, 
      but little is known of their possible functions in DC biology. We demonstrate 
      that the inhibitory receptor LILRB1 (ILT2, LIR1, CD85j) is selectively 
      up-regulated during DC differentiation from monocyte precursors in culture. 
      Continuous ligation of LILRB1 modulated cellular differentiation, conferred a 
      unique phenotype upon the resultant cells, induced a profound resistance to 
      CD95-mediated cell death, and inhibited secretion of cytokines IL-10, IL-12p70, 
      and TGF-beta. These features remained stable even after exposure of the cells to 
      bacterial LPS. Ligated DCs exhibited poor stimulatory activity for primary and 
      memory T-cell proliferative responses, but this was substantially reversed by 
      blockade of CD80 or its preferred ligand CTLA-4, or by depleting CD4(+) CD25(+) 
      CD127(lo) regulatory T cells. Our findings suggest that ligation of LILRB1 on DCs 
      by self-HLA molecules may play a key role in controlling the balance between the 
      induction and suppression of adaptive immune responses.
FAU - Young, Neil T
AU  - Young NT
AD  - Division of Immunology, Department of Pathology, University of Cambridge, 
      Cambridge, UK. nty20@cam.ac.uk
FAU - Waller, Edward C P
AU  - Waller EC
FAU - Patel, Rashmi
AU  - Patel R
FAU - Roghanian, Ali
AU  - Roghanian A
FAU - Austyn, Jonathan M
AU  - Austyn JM
FAU - Trowsdale, John
AU  - Trowsdale J
LA  - eng
GR  - G0401569/MRC_/Medical Research Council/United Kingdom
GR  - G9800943/MRC_/Medical Research Council/United Kingdom
GR  - WT_/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071219
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, CD)
RN  - 0 (Cytokines)
RN  - 0 (LILRB1 protein, human)
RN  - 0 (Leukocyte Immunoglobulin-like Receptor B1)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (fas Receptor)
RN  - 21820-51-9 (Phosphotyrosine)
SB  - IM
MH  - Antigens, CD/*metabolism
MH  - Cell Differentiation/*drug effects/*immunology
MH  - Cells, Cultured
MH  - Cytokines/metabolism
MH  - Dendritic Cells/*cytology/drug effects/immunology/*metabolism
MH  - Humans
MH  - Immunologic Memory/immunology
MH  - Leukocyte Immunoglobulin-like Receptor B1
MH  - Lipopolysaccharides/pharmacology
MH  - Phenotype
MH  - Phosphotyrosine/metabolism
MH  - Receptors, Immunologic/*antagonists & inhibitors/*metabolism
MH  - fas Receptor/immunology
EDAT- 2007/12/21 09:00
MHDA- 2008/04/26 09:00
CRDT- 2007/12/21 09:00
PHST- 2007/12/21 09:00 [pubmed]
PHST- 2008/04/26 09:00 [medline]
PHST- 2007/12/21 09:00 [entrez]
AID - S0006-4971(20)48618-5 [pii]
AID - 10.1182/blood-2007-05-089771 [doi]
PST - ppublish
SO  - Blood. 2008 Mar 15;111(6):3090-6. doi: 10.1182/blood-2007-05-089771. Epub 2007 
      Dec 19.