PMID- 18094413
OWN - NLM
STAT- MEDLINE
DCOM- 20080304
LR  - 20071220
IS  - 1078-0432 (Print)
IS  - 1078-0432 (Linking)
VI  - 13
IP  - 24
DP  - 2007 Dec 15
TI  - EWSR1-CREB1 and EWSR1-ATF1 fusion genes in angiomatoid fibrous histiocytoma.
PG  - 7322-8
AB  - PURPOSE: Angiomatoid fibrous histiocytoma (AFH) is a low-grade mesenchymal 
      neoplasm which usually occurs in children and adolescents. Either FUS-ATF1 or 
      EWSR1-ATF1 have been detected in the few cases published, pointing to the 
      interchangeable role of FUS and EWSR1 in this entity. EWSR1-ATF1 also represents 
      the most frequent genetic alteration in clear cell sarcoma, suggesting the 
      existence of a molecular homology between these two histotypes. We investigated 
      the presence of EWSR1-CREB1, recently found in gastrointestinal clear cell 
      sarcoma, and FUS-CREB1, as well as the already reported FUS-ATF1 and EWSR1-ATF1 
      in a series of AFH. EXPERIMENTAL DESIGN: Fourteen cases were analyzed by 
      fluorescence in situ hybridization (FISH) on paraffin-embedded tissue sections, 
      using a commercial EWSR1 probe and custom-designed probes for FUS, ATF1, and 
      CREB1. In two cases, four-color FISH was also done. Reverse transcription-PCR for 
      the four hypothetical fusion genes was done in one case, for which frozen 
      material was available. RESULTS: Thirteen cases showed rearrangements of both 
      EWSR1 and CREB1, whereas one case showed the rearrangement of both EWSR1 and 
      ATF1. Four-color FISH confirmed the results in two selected cases. Reverse 
      transcription-PCR showed EWSR1-CREB1 transcript in the case analyzed. CONCLUSION: 
      We identified the presence of either EWSR1-CREB1 or EWSR1-ATF1 in all the cases, 
      strengthening the concept of chromosomal promiscuity between AFH and clear cell 
      sarcoma. Either the occurrence of a second unknown tumor-specific molecular event 
      or, perhaps more likely, divergent differentiation programs of the putatively 
      distinct precursor cells of AFH and clear cell sarcoma might be invoked in order 
      to explain the two different phenotypes.
FAU - Rossi, Sabrina
AU  - Rossi S
AD  - Department of Pathology, Regional Hospital, Treviso, Italy.
FAU - Szuhai, Karoly
AU  - Szuhai K
FAU - Ijszenga, Marije
AU  - Ijszenga M
FAU - Tanke, Hans J
AU  - Tanke HJ
FAU - Zanatta, Lucia
AU  - Zanatta L
FAU - Sciot, Raf
AU  - Sciot R
FAU - Fletcher, Christopher D M
AU  - Fletcher CD
FAU - Dei Tos, Angelo P
AU  - Dei Tos AP
FAU - Hogendoorn, Pancras C W
AU  - Hogendoorn PC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Clin Cancer Res
JT  - Clinical cancer research : an official journal of the American Association for 
      Cancer Research
JID - 9502500
RN  - 0 (CREB1 protein, human)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (EWS-ATF1 fusion protein, human)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Base Sequence
MH  - Child
MH  - Child, Preschool
MH  - Cyclic AMP Response Element-Binding Protein/*genetics
MH  - Female
MH  - Histiocytoma, Malignant Fibrous/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Oncogene Proteins, Fusion/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Soft Tissue Neoplasms/*genetics
MH  - Transcription Factors/*genetics
EDAT- 2007/12/21 09:00
MHDA- 2008/03/05 09:00
CRDT- 2007/12/21 09:00
PHST- 2007/12/21 09:00 [pubmed]
PHST- 2008/03/05 09:00 [medline]
PHST- 2007/12/21 09:00 [entrez]
AID - 13/24/7322 [pii]
AID - 10.1158/1078-0432.CCR-07-1744 [doi]
PST - ppublish
SO  - Clin Cancer Res. 2007 Dec 15;13(24):7322-8. doi: 10.1158/1078-0432.CCR-07-1744.