PMID- 18094995
OWN - NLM
STAT- MEDLINE
DCOM- 20080730
LR  - 20211020
IS  - 0945-6317 (Print)
IS  - 0945-6317 (Linking)
VI  - 452
IP  - 3
DP  - 2008 Mar
TI  - Advantage of FISH analysis using FKHR probes for an adjunct to diagnosis of 
      rhabdomyosarcomas.
PG  - 251-8
AB  - Translocations can be detected using fluorescence in situ hybridization (FISH) in 
      formalin-fixed paraffin-embedded tissues. Recently, a commercially available FKHR 
      (13q14) dual-color, break-apart rearrangement probe has been developed. However, 
      the advantages of using this probe have not been reported. This study 
      demonstrated the usefulness of this probe for the clinical diagnosis of 
      rhabdomyosarcomas (RMS). We studied 33 RMS (19 embryonal rhabdomyosarcomas 
      [ERMS], including three sclerosing-type RMS, and 14 alveloar rhabdomyosarcomas 
      [ARMS]). Fluorescence signals were detected for 18 of the 19 (94.7%) ERMS and 13 
      of the 14 (92.8%) ARMS. A split-signal pattern was detected in 12 of 13 (92.3%) 
      ARMS but was not detected in any of the ERMS, including the three sclerosing-type 
      RMS. Amplification and polyploidy were present in both the ERMS and the ARMS. Our 
      FISH study highlighted the excellent performance of the presently reported 
      commercial break-apart probe for the detection of FKHR gene rearrangements in 
      RMS. Because amplification and polyploidy were detected in both the ERMS and the 
      ARMS, sufficient care should be taken when counting the nuclear signals. No 
      rearrangements of the FKHR gene were found in any of the three sclerosing-type 
      RMS when examined using a FISH assay, supporting the hypothesis that sclerosing 
      RMS can be included as an ERMS.
FAU - Matsumura, Tadaki
AU  - Matsumura T
AD  - Department of Surgical Pathology, Sapporo Medical University School of Medicine, 
      South 1, West 16, Chuo-ku, Sapporo 060-8543, Japan.
FAU - Yamaguchi, Takehiko
AU  - Yamaguchi T
FAU - Seki, Kunihiko
AU  - Seki K
FAU - Shimoda, Tadakazu
AU  - Shimoda T
FAU - Wada, Takuro
AU  - Wada T
FAU - Yamashita, Toshihiko
AU  - Yamashita T
FAU - Hasegawa, Tadashi
AU  - Hasegawa T
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Virchows Arch
JT  - Virchows Archiv : an international journal of pathology
JID - 9423843
RN  - 0 (DNA Probes)
RN  - 0 (FOXO1 protein, human)
RN  - 0 (Forkhead Box Protein O1)
RN  - 0 (Forkhead Transcription Factors)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Child
MH  - Child, Preschool
MH  - DNA Probes/genetics
MH  - Female
MH  - Forkhead Box Protein O1
MH  - Forkhead Transcription Factors/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Infant
MH  - Male
MH  - Middle Aged
MH  - Reproducibility of Results
MH  - Rhabdomyosarcoma/*diagnosis/genetics
MH  - Sensitivity and Specificity
EDAT- 2007/12/21 09:00
MHDA- 2008/07/31 09:00
CRDT- 2007/12/21 09:00
PHST- 2007/05/23 00:00 [received]
PHST- 2007/11/29 00:00 [accepted]
PHST- 2007/11/05 00:00 [revised]
PHST- 2007/12/21 09:00 [pubmed]
PHST- 2008/07/31 09:00 [medline]
PHST- 2007/12/21 09:00 [entrez]
AID - 10.1007/s00428-007-0554-9 [doi]
PST - ppublish
SO  - Virchows Arch. 2008 Mar;452(3):251-8. doi: 10.1007/s00428-007-0554-9.