PMID- 18095589
OWN - NLM
STAT- MEDLINE
DCOM- 20100520
LR  - 20071221
IS  - 1672-173X (Print)
IS  - 1672-173X (Linking)
VI  - 38
IP  - 6
DP  - 2007 Nov
TI  - [Signaling pathway of BDNF protecting embryonic cortical neurons from hypoxia].
PG  - 934-7
AB  - OBJECTIVE: To investigate the changes of TrkB and Ras-MAPK in brain-derived 
      neurotrophic factor (BDNF) protecting the embryonic rat cerebral cortical neurons 
      against hypoxia-induced neurotoxicity. METHODS: The immunofluorescence technique, 
      laser scanning confocal microscope and half-quantitative analysis were done to 
      explore the changes of the intracellular levels of tyrosine kinase B (TrkB), 
      phosphorylated TrkB and the mitogen-activated protein kinase (MAPK) in rat 
      embryonic cortical neurons cultured in different time groups of hypoxia with or 
      without BDNF pretreatment. RESULTS: The fluorescent intensity of TrkB and 
      phosphorylated TrkB in the cytoplasm and the fluorescent intensity of MAPK in 
      both cytoplasm and cell nucleus of the neurons were significantly increased in 
      the presence of BDNF (P < 0.05), the fluorescent intensity of neurons pretreated 
      with BDNF 24 h before the hypoxia culture was stronger than those with BDNF just 
      before the hypoxia culture (P < 0.05). CONCLUSION: The Ras-MAPK approach may be 
      the major signal transferring way of BDNF in protecting the cortical neurons from 
      hypoxia-induced neurotoxicity. The approach of signal transferring begins with 
      tyrosine phosphorylation of TrkB receptors.
FAU - Wang, Zhi-ling
AU  - Wang ZL
AD  - Department of Pediatrics, West China Second Hospital, Sichuan University, 
      Chengdu, China.
FAU - Mao, Meng
AU  - Mao M
FAU - Zhou, Hui
AU  - Zhou H
FAU - Li, Sheng-fu
AU  - Li SF
FAU - Yu, Dan
AU  - Yu D
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Sichuan Da Xue Xue Bao Yi Xue Ban
JT  - Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical 
      science edition
JID - 101162609
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 3.6.5.2 (ras Proteins)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cell Hypoxia
MH  - Cells, Cultured
MH  - Cerebral Cortex/*cytology
MH  - Mitogen-Activated Protein Kinases/metabolism
MH  - Neurons/*metabolism
MH  - Phosphorylation
MH  - Rats
MH  - Receptor, trkB/metabolism
MH  - *Signal Transduction
MH  - ras Proteins/metabolism
EDAT- 2007/12/22 09:00
MHDA- 2010/05/21 06:00
CRDT- 2007/12/22 09:00
PHST- 2007/12/22 09:00 [pubmed]
PHST- 2010/05/21 06:00 [medline]
PHST- 2007/12/22 09:00 [entrez]
PST - ppublish
SO  - Sichuan Da Xue Xue Bao Yi Xue Ban. 2007 Nov;38(6):934-7.