PMID- 18097181
OWN - NLM
STAT- MEDLINE
DCOM- 20080206
LR  - 20171116
IS  - 1423-0267 (Electronic)
IS  - 0030-3755 (Linking)
VI  - 222
IP  - 1
DP  - 2008
TI  - Culture in vitro modulation of human leukocyte antigen molecules and 
      costimulatory molecules on human retinal pigment epithelium.
PG  - 48-52
AB  - PURPOSE: To determine the potential of culture in vitro to alter the human 
      leukocyte antigen (HLA) molecules and costimulatory molecules on human retinal 
      pigment epithelium (RPE). METHODS: Pure RPE were isolated and cultured. Two sets 
      of RPE (normal and activated) were used. Activated RPE were obtained by 
      incubating primary cultures of RPE with recombinant human interferon-gamma. The 
      expression of HLA molecules and costimulatory molecules on human RPE at different 
      passages after culture in vitro were analyzed quantitatively by flow cytometry. 
      RESULTS: In the process of routine culture on culture flask, the duration of 
      culture in vitro was significantly correlated with the expression of HLA-ABC 
      molecules on the normal RPE and the activated RPE (r = -0.893, p < 0.001 and r = 
      -0.964, p < 0.001 respectively), HLA-DR molecule on the activated RPE (r = 
      -0.901, p < 0.001) and intercellular adhesion molecule-1 on the normal RPE (r = 
      0.961, p < 0.001). There were no correlations between the duration of culture in 
      vitro and the expression of HLA-DR molecule on the normal RPE, intercellular 
      adhesion molecule-1 on the activated RPE, B7-1 and B7-2 molecules on the normal 
      RPE and the activated RPE. CONCLUSIONS: The chronic rejection is the major 
      immunological rejection following RPE allogeneic graft. Culture in vitro 
      modulation of HLA molecules and costimulatory molecules on RPE may increase 
      lymphocyte infiltration and the activated RPE.
CI  - (c) 2007 S. Karger AG, Basel.
FAU - Xie, Mao-Song
AU  - Xie MS
AD  - Department of Ophthalmology, School of Medicine, Shandong University, Jinan, 
      China.
FAU - Xu, Guo-Xing
AU  - Xu GX
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071219
PL  - Switzerland
TA  - Ophthalmologica
JT  - Ophthalmologica. Journal international d'ophtalmologie. International journal of 
      ophthalmology. Zeitschrift fur Augenheilkunde
JID - 0054655
RN  - 0 (Antigens, Surface)
RN  - 0 (B7-1 Antigen)
RN  - 0 (B7-2 Antigen)
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Recombinant Proteins)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Antigens, Surface/*metabolism
MH  - B7-1 Antigen
MH  - B7-2 Antigen
MH  - Cells, Cultured
MH  - Flow Cytometry
MH  - Graft Rejection/immunology
MH  - HLA Antigens/*metabolism
MH  - HLA-DR Antigens/metabolism
MH  - Humans
MH  - In Vitro Techniques
MH  - Intercellular Adhesion Molecule-1/metabolism
MH  - Interferon-gamma/*pharmacology
MH  - Lymphocyte Activation/immunology
MH  - Lymphocytosis/immunology
MH  - Pigment Epithelium of Eye/drug effects/immunology/*physiology/transplantation
MH  - Recombinant Proteins/pharmacology
EDAT- 2007/12/22 09:00
MHDA- 2008/02/07 09:00
CRDT- 2007/12/22 09:00
PHST- 2007/12/22 09:00 [pubmed]
PHST- 2008/02/07 09:00 [medline]
PHST- 2007/12/22 09:00 [entrez]
AID - 000109279 [pii]
AID - 10.1159/000109279 [doi]
PST - ppublish
SO  - Ophthalmologica. 2008;222(1):48-52. doi: 10.1159/000109279. Epub 2007 Dec 19.