PMID- 18097567 OWN - NLM STAT- MEDLINE DCOM- 20080320 LR - 20171116 IS - 1019-6439 (Print) IS - 1019-6439 (Linking) VI - 32 IP - 1 DP - 2008 Jan TI - Live cell vaccines expressing B7.1, monocyte chemoattractant protein 1 and granulocyte-macrophage colony stimulation factor derived from mouse HPV16-transformed cells. PG - 265-71 AB - One of the gene therapy strategies in oncology is immunization with cancer cells that express various cytokines. We used a thymidine-kinase deficient (cTK-) cell line designated 123IA, which had been derived from HPV16-transformed mouse (C57BL/6) cells MK16/I/III/ABC (MK16). To obtain genetically modified cells, 123IA cells were transfected with bicistronic plasmid vectors carrying the herpes simplex type 1 thymidine kinase (HSV TK) gene and either the gene for the mouse B7.1 (CD80) co-stimulatory molecule or the gene for the monocyte-chemoattractant protein 1 (MCP-1). For control purposes, a plasmid vector carrying only the HSV TK gene was used. The transfected cells were cultivated in medium supplemented with hypoxanthine, aminopterin and thymidine. For comparative purposes we also used B9 cells, which express the granulocyte-macrophage colony stimulation factor (GM-CSF) and had been derived from 123A cells by transduction with the recombinant adeno-associated virus carrying the HSV TK gene and the mouse GM-CSF gene. All of the cell lines isolated were found to be sensitive to minute amounts of ganciclovir, revealing the production of HSV TK, and to express the respective transgenes. When inoculated into 5-week-old female syngeneic mice, cells expressing either GM-CSF or B7.1 were non-oncogenic. On the other hand, nearly all mice inoculated with MCP-1-producing cells developed tumours, though considerably later than animals inoculated with the same dose of the parental MK16 cells. Animals injected with GM-CSF- or B7.1-producing cells were protected against challenge with the parental MK16 cells. When another mouse (C57BL/6) HPV16-transformed oncogenic cell line, TC-1, which differs from the MK16 cells in a number of properties such as MHC class I and B7.1 expression, was used for the challenge, the protective effect was much less pronounced. FAU - Lakatosova-Andelova, Monika AU - Lakatosova-Andelova M AD - Department of Experimental Virology, Institute of Haematology and Blood Transfusion, Prague, The Czech Republic. FAU - Jinoch, Pavel AU - Jinoch P FAU - Duskova, Martina AU - Duskova M FAU - Marinov, Iuri AU - Marinov I FAU - Vonka, Vladimir AU - Vonka V LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Greece TA - Int J Oncol JT - International journal of oncology JID - 9306042 RN - 0 (B7-1 Antigen) RN - 0 (Cancer Vaccines) RN - 0 (Ccl2 protein, mouse) RN - 0 (Chemokine CCL2) RN - 0 (E6 protein, Human papillomavirus type 16) RN - 0 (Oncogene Proteins, Viral) RN - 0 (Papillomavirus E7 Proteins) RN - 0 (Repressor Proteins) RN - 0 (oncogene protein E7, Human papillomavirus type 16) RN - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor) RN - P9G3CKZ4P5 (Ganciclovir) SB - IM MH - Animals MH - B7-1 Antigen/*genetics MH - Cancer Vaccines/*immunology MH - Cell Line MH - Cell Line, Transformed MH - Chemokine CCL2/*genetics MH - Female MH - Ganciclovir/pharmacology MH - Genes, MHC Class I MH - Granulocyte-Macrophage Colony-Stimulating Factor/*genetics MH - Human papillomavirus 16/*genetics MH - Immunization MH - Mice MH - Mice, Inbred C57BL MH - Oncogene Proteins, Viral/genetics MH - Papillomavirus E7 Proteins MH - Repressor Proteins/genetics MH - Transfection EDAT- 2007/12/22 09:00 MHDA- 2008/03/21 09:00 CRDT- 2007/12/22 09:00 PHST- 2007/12/22 09:00 [pubmed] PHST- 2008/03/21 09:00 [medline] PHST- 2007/12/22 09:00 [entrez] PST - ppublish SO - Int J Oncol. 2008 Jan;32(1):265-71.