PMID- 18097851
OWN - NLM
STAT- MEDLINE
DCOM- 20080327
LR  - 20191210
IS  - 0265-6736 (Print)
IS  - 0265-6736 (Linking)
VI  - 23
IP  - 8
DP  - 2007 Dec
TI  - Hyperthermia induces differentiation without apoptosis in permissive temperatures 
      in human erythroleukaemia cells.
PG  - 645-55
AB  - PURPOSE: The aim of the present study was to investigate whether induction of 
      differentiation by hyperthermia is accompanied by apoptosis and necrosis to 
      further evaluate the benefits of using hyperthermia as a differentiation inducing 
      physical modality. MATERIALS AND METHOD: Differentiation was evaluated in K562 
      erythroleukaemia cells by measuring haemoglobin synthesis and flow cytometric 
      measurement of glycophorin A expression. Apoptosis was measured by Annexin-V-FITC 
      and Propidium Iodide (PI) double staining assay. Apoptosis and necrosis was also 
      evaluated morphologically using staining with acridine orange/ethidium bromide 
      (AO/EtBr) by fluorescence microscopy. Heat shock protein 70 (HSP70) level was 
      measured by ELISA kit. RESULTS: Hyperthermia (43 degrees C) induced 
      differentiation as judged by increased haemoglobin synthesis and glycophorin A 
      expression. No sign of apoptosis or necrosis could be detected at this 
      temperature. Cell viability did not change due to heat treatment, and cellular 
      proliferation was reduced in a dose (heating time) dependent manner. At 45 
      degrees C, hyperthermia induced apoptosis and necrosis with minimal or no sign of 
      differentiation. HSP70 level was significantly increased at 43 degrees C along 
      with differentiation of leukaemic cells, while at 45 degrees C no significant 
      effect on HSP70 production could be observed. CONCLUSIONS: The encouraging 
      results obtained here indicate that by heat treatment at 43 degrees C, 
      hyperthermia can be used alone or in combination with other modalities as a 
      differentiation inducing agent without any detectable apoptotic activity. 
      Positive correlation between HSP70 production and induction of differentiation 
      and lack of apoptosis by hyperthermia confirm the possible role of HSP70 in the 
      heat-induced differentiation and apoptosis in leukaemic cells.
FAU - Sharif-Khatibi, Leila
AU  - Sharif-Khatibi L
AD  - Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
FAU - Kariminia, Amina
AU  - Kariminia A
FAU - Khoei, Sameide
AU  - Khoei S
FAU - Goliaei, Bahram
AU  - Goliaei B
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int J Hyperthermia
JT  - International journal of hyperthermia : the official journal of European Society 
      for Hyperthermic Oncology, North American Hyperthermia Group
JID - 8508395
RN  - 0 (HSP70 Heat-Shock Proteins)
SB  - IM
MH  - Apoptosis/*physiology
MH  - Cell Differentiation/*physiology
MH  - Cell Proliferation
MH  - Cell Survival
MH  - Fever/metabolism/pathology
MH  - HSP70 Heat-Shock Proteins/metabolism
MH  - *Hot Temperature
MH  - Humans
MH  - K562 Cells
MH  - Leukemia, Erythroblastic, Acute/metabolism/*pathology
MH  - Temperature
EDAT- 2007/12/22 09:00
MHDA- 2008/03/28 09:00
CRDT- 2007/12/22 09:00
PHST- 2007/12/22 09:00 [pubmed]
PHST- 2008/03/28 09:00 [medline]
PHST- 2007/12/22 09:00 [entrez]
AID - 788717847 [pii]
AID - 10.1080/02656730701769833 [doi]
PST - ppublish
SO  - Int J Hyperthermia. 2007 Dec;23(8):645-55. doi: 10.1080/02656730701769833.