PMID- 181516
OWN - NLM
STAT- MEDLINE
DCOM- 19761002
LR  - 20181130
IS  - 0022-0795 (Print)
IS  - 0022-0795 (Linking)
VI  - 69
IP  - 3
DP  - 1976 Jun
TI  - Effect of guanyl nucleotides on parathyroid hormone-responsive adenylate cyclase 
      in chick kidney.
PG  - 401-12
AB  - Both guanosine 5'-triphosphate (GTP) AND 5'-guanylylimidodiphosphate (Gpp(NH)p) 
      activated adenylate cyclase (EC4.6.1.1) in chick kidney plasma membranes. 
      Half-maximal stimulation occurred at 3-1 X 10(-6)M for both agents. The maximum 
      increases in adenylate cyclase activity produced by GTP and Gpp(NH)p were 
      respectively 130 and 720% over basal activity. At the end of a 12 min incubation 
      period GTP concentration was 85% of that originally added in the presence of an 
      ATP-regenerating system but less than 20% in its absence. GTP and guanosine 
      5'-diphosphate inhibited the activation of adenylate cyclase by Gpp(NH)p, 
      suggesting that they all acted at a common site. Gpp(NH)p facilitated the 
      stimulation of adenylate cyclase activity by bovine parathyroid hormone (BPTH) 
      and by the synthetic amino terminal fragment BPTH (1-34), decreasing the 
      concentrations required for half-maximal enzyme activation by a factor of 
      approximately eight in both cases. This property was not shared by the native 
      nucleotide GTP. Gpp(NH)p rendered active (at certain concentrations) a synthetic 
      parathyroid hormone peptide fragment, BPTH (2-34), which was incapable of 
      Activating adenylate cyclase in the absence of the nucleotide analogue. This 
      suggested that the GTP analogue, in addition to a direct effect upon adenylate 
      cyclase activity, was capable of influencing hormone interaction with the enzyme 
      complex.
FAU - Hunt, N H
AU  - Hunt NH
FAU - Martin, T J
AU  - Martin TJ
FAU - Michelangeli, V P
AU  - Michelangeli VP
FAU - Eisman, J A
AU  - Eisman JA
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Endocrinol
JT  - The Journal of endocrinology
JID - 0375363
RN  - 0 (Guanine Nucleotides)
RN  - 0 (Parathyroid Hormone)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 4.6.1.1 (Adenylyl Cyclases)
SB  - IM
MH  - Adenylyl Cyclases/*metabolism
MH  - Animals
MH  - Cell Membrane/enzymology
MH  - Chickens
MH  - Cyclic AMP/metabolism
MH  - Enzyme Activation
MH  - Guanine Nucleotides/*pharmacology
MH  - Guanosine Triphosphate/*pharmacology
MH  - Kidney/drug effects/*enzymology
MH  - Male
MH  - Parathyroid Hormone/*pharmacology
EDAT- 1976/06/01 00:00
MHDA- 1976/06/01 00:01
CRDT- 1976/06/01 00:00
PHST- 1976/06/01 00:00 [pubmed]
PHST- 1976/06/01 00:01 [medline]
PHST- 1976/06/01 00:00 [entrez]
PST - ppublish
SO  - J Endocrinol. 1976 Jun;69(3):401-12.