PMID- 18154518
OWN - NLM
STAT- MEDLINE
DCOM- 20080425
LR  - 20200930
IS  - 1536-2302 (Print)
IS  - 1536-2302 (Linking)
VI  - 9
IP  - 4
DP  - 2007 Winter
TI  - Ex vivo characteristics of human amniotic membrane-derived stem cells.
PG  - 581-94
AB  - Cells were isolated from four human amniotic membranes, and their biological 
      characteristics analyzed during ex vivo expansion. Morphologically homogenous 
      populations of fibroblast-like cells were obtained from the second or third 
      passage. Under the appropriate culture conditions, these human amniotic 
      membrane-derived mesenchymal cells (HAM) were shown to differentiate into 
      adipocytes, osteocytes, chondrocytes and neuronal cells, as visualized by Oil Red 
      O, von Kossa, alcian blue, anti-Neu N, and anti-Gal C antibody staining, 
      respectively. Immunophenotype analysis of HAM cells revealed the presence of 
      antigens for SSEA-3, SSEA-4, collagen type-I, -II, -III, -IV, -XII, fibronectin, 
      alpha-SMA, vimentin, desmin, cytokeratin18 (CK18), HCAM-1, fibroblast surface 
      protein, and human leukocyte antigen (HLA) ABC. ICAM-1 protein was weakly 
      detectable, and proteins of TRA-1-60, VCAM-1, von Willebrand factor, PECAM-1, and 
      HLA DR were not detected. HAM cells reached senescence after 14.5+/-0.9 passages, 
      over a period of 146.8+/-8.9 days, and underwent an average of 36.9 4.7 
      population doublings. RT-PCR analysis showed that all four HAM cell lines 
      consistently expressed genes of Oct-4, Rex-1, SCF, NCAM, nestin, BMP-4, GATA-4, 
      HNF-4alpha, vimentin, and CK18, regardless of the passage number. The genes of 
      Brachyury, FGF-5, Pax-6, and BMP2 were never expressed. Strikingly, 
      alpha-fetoprotein (alphaFP), HLA ABC, and HLA DR genes were expressed in an 
      earlier passage but not expressed in later passages. Telomerase activity of two 
      HAM lines was discernable upon the third passage. These observations strongly 
      suggest that HAM might be immune-privileged and, thus, advantageous as 
      therapeutic cells.
FAU - Kim, JiYoung
AU  - Kim J
AD  - Department of Biotechnology, College of Natural Sciences, Seoul Women's 
      University, Nowon-gu, Seoul, Korea. toelmo@hanmail.net
FAU - Kang, Hyun Mi
AU  - Kang HM
FAU - Kim, Haekwon
AU  - Kim H
FAU - Kim, Mee Ran
AU  - Kim MR
FAU - Kwon, Hyuck Chan
AU  - Kwon HC
FAU - Gye, Myung Chan
AU  - Gye MC
FAU - Kang, Sung Goo
AU  - Kang SG
FAU - Yang, H Seung
AU  - Yang HS
FAU - You, Juice
AU  - You J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cloning Stem Cells
JT  - Cloning and stem cells
JID - 101125444
RN  - 0 (Culture Media)
RN  - 0 (DNA Primers)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Amnion/*cytology
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - Culture Media/pharmacology
MH  - DNA Primers/chemistry
MH  - Gene Expression Profiling
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Immunophenotyping
MH  - Mesoderm/*cytology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Stem Cells/*cytology
MH  - Telomerase/metabolism
EDAT- 2007/12/25 09:00
MHDA- 2008/04/26 09:00
CRDT- 2007/12/25 09:00
PHST- 2007/12/25 09:00 [pubmed]
PHST- 2008/04/26 09:00 [medline]
PHST- 2007/12/25 09:00 [entrez]
AID - 10.1089/clo.2007.0027 [doi]
PST - ppublish
SO  - Cloning Stem Cells. 2007 Winter;9(4):581-94. doi: 10.1089/clo.2007.0027.