PMID- 18154977
OWN - NLM
STAT- MEDLINE
DCOM- 20080325
LR  - 20191210
IS  - 0021-9673 (Print)
IS  - 0021-9673 (Linking)
VI  - 1179
IP  - 2
DP  - 2008 Feb 1
TI  - Molecularly imprinted capillary electrochromatography for selective determination 
      of thiabendazole in citrus samples.
PG  - 216-23
AB  - In this work, the suitability of the combination of molecular imprinting and 
      capillary electrochromatography (MIP-CEC) to be used as powerful tool in 
      environmental or food analysis has been for the first time studied and 
      successfully demonstrated. A molecularly imprinted monolith (MIM) has been 
      synthesised and evaluated as stationary phase for the selective determination of 
      the fungicide thiabendazole (TBZ) in citrus samples by non-aqueous capillary 
      electrochromatography. The influence of the mobile phase composition, the voltage 
      of the power supply and the separation temperature on the recognition of TBZ by 
      the imprinted polymer has been evaluated, and the imprint effect in the MIM was 
      clearly demonstrated. Once optimum recognition conditions were established, other 
      variables affecting mechanical properties and chromatographic performance of MIM 
      were adjusted using computational approach. The high selectivity achieved by the 
      MIP-CEC developed procedure allowed unambiguous detection and quantification of 
      TBZ in citrus samples by direct injection of the crude sample extracts, without 
      any previous clean-up, in less than 6 min. The developed method was properly 
      validated and the calculated detection limits were below the established maximum 
      residue limits (MRLs), clearly demonstrating the suitability of the method to be 
      used for the control of the selected fungicide.
FAU - Cacho, Carmen
AU  - Cacho C
AD  - Departamento de Quimica Analitica, Facultad de Ciencias Quimicas, Universidad 
      Complutense de Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain.
FAU - Schweitz, Leif
AU  - Schweitz L
FAU - Turiel, Esther
AU  - Turiel E
FAU - Perez-Conde, Concepcion
AU  - Perez-Conde C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20071203
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - N1Q45E87DT (Thiabendazole)
SB  - IM
MH  - Capillary Electrochromatography/instrumentation/*methods
MH  - Chromatography, High Pressure Liquid/methods
MH  - Citrus/*chemistry
MH  - Food Contamination/analysis
MH  - Molecular Imprinting/*instrumentation
MH  - Reproducibility of Results
MH  - Thiabendazole/*analysis
EDAT- 2007/12/25 09:00
MHDA- 2008/03/26 09:00
CRDT- 2007/12/25 09:00
PHST- 2007/09/14 00:00 [received]
PHST- 2007/11/09 00:00 [revised]
PHST- 2007/11/28 00:00 [accepted]
PHST- 2007/12/25 09:00 [pubmed]
PHST- 2008/03/26 09:00 [medline]
PHST- 2007/12/25 09:00 [entrez]
AID - S0021-9673(07)02101-2 [pii]
AID - 10.1016/j.chroma.2007.11.097 [doi]
PST - ppublish
SO  - J Chromatogr A. 2008 Feb 1;1179(2):216-23. doi: 10.1016/j.chroma.2007.11.097. 
      Epub 2007 Dec 3.