PMID- 18155882
OWN - NLM
STAT- MEDLINE
DCOM- 20080506
LR  - 20191210
IS  - 0924-8579 (Print)
IS  - 0924-8579 (Linking)
VI  - 31
IP  - 3
DP  - 2008 Mar
TI  - A novel structure of Tn4001-truncated element, type V, in clinical enterococcal 
      isolates and multiplex PCR for detecting aminoglycoside resistance genes.
PG  - 250-4
AB  - A multiplex polymerase chain reaction (PCR) was established for detecting 
      aacA-aphD, aph(2'')-Ib, aph(2'')-Ic and aph(2'')-Id, encoding high-level 
      gentamicin resistance (HLGR), and aadA and aadE, encoding high-level streptomycin 
      resistance (HLSR), in enterococci. The assay was implemented for 419 enterococcal 
      blood and urine isolates recovered from patients at a university hospital in 
      Thailand. Among the isolates tested, 56.1% (235 isolates) and 58.9% (247 
      isolates) contained aacA-aphD and aadE, respectively. The aph(2'')-Ib, 
      aph(2'')-Ic, aph(2'')-Id and aadA genes were not found in any isolate. Among the 
      isolates carrying the aacA-aphD gene, 99.1% exhibited a HLGR phenotype. All 235 
      enterococcal isolates containing aacA-aphD were further studied by PCR to 
      characterise the structure of the resistance determinants carrying the aacA-aphD 
      gene. The result revealed that only 22.6% carried Tn4001-related element, whereas 
      the remaining isolates contained Tn4001-truncated element. No Tn4001-IS257 hybrid 
      structure was detected. The majority of isolates carrying Tn4001-related element 
      were Enterococcus faecalis (77.4%). Among Tn4001-truncated elements detected, all 
      previously reported types (types I-IV) were found. Furthermore, a novel 
      Tn4001-truncated type, designated type V, was also identified.
FAU - Leelaporn, A
AU  - Leelaporn A
AD  - Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol 
      University, Bangkoknoi, Bangkok 10700, Thailand. siale@mucc.mahidol.ac.th
FAU - Yodkamol, K
AU  - Yodkamol K
FAU - Waywa, D
AU  - Waywa D
FAU - Pattanachaiwit, S
AU  - Pattanachaiwit S
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071226
PL  - Netherlands
TA  - Int J Antimicrob Agents
JT  - International journal of antimicrobial agents
JID - 9111860
RN  - 0 (Aminoglycosides)
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA Transposable Elements)
RN  - 0 (DNA, Bacterial)
SB  - IM
MH  - Aminoglycosides/*pharmacology
MH  - Anti-Bacterial Agents/*pharmacology
MH  - Bacterial Proteins/genetics
MH  - Blood/microbiology
MH  - *DNA Transposable Elements
MH  - DNA, Bacterial/genetics
MH  - Drug Resistance, Bacterial/*genetics
MH  - Enterococcus/drug effects/*genetics/isolation & purification
MH  - Gram-Positive Bacterial Infections/microbiology
MH  - Humans
MH  - Polymerase Chain Reaction/*methods
MH  - Thailand
MH  - Urine/microbiology
EDAT- 2007/12/25 09:00
MHDA- 2008/05/07 09:00
CRDT- 2007/12/25 09:00
PHST- 2007/08/03 00:00 [received]
PHST- 2007/10/11 00:00 [revised]
PHST- 2007/10/13 00:00 [accepted]
PHST- 2007/12/25 09:00 [pubmed]
PHST- 2008/05/07 09:00 [medline]
PHST- 2007/12/25 09:00 [entrez]
AID - S0924-8579(07)00543-2 [pii]
AID - 10.1016/j.ijantimicag.2007.10.019 [doi]
PST - ppublish
SO  - Int J Antimicrob Agents. 2008 Mar;31(3):250-4. doi: 
      10.1016/j.ijantimicag.2007.10.019. Epub 2007 Dec 26.