PMID- 18156650
OWN - NLM
STAT- MEDLINE
DCOM- 20080317
LR  - 20080229
IS  - 1460-2350 (Electronic)
IS  - 0268-1161 (Linking)
VI  - 23
IP  - 3
DP  - 2008 Mar
TI  - Proteomic analysis of human omental adipose tissue in the polycystic ovary 
      syndrome using two-dimensional difference gel electrophoresis and mass 
      spectrometry.
PG  - 651-61
AB  - BACKGROUND: Our aim was to study the protein expression profiles of omental 
      adipose tissue biopsies obtained from morbidly obese women with or without 
      polycystic ovary syndrome (PCOS) at the time of bariatric surgery to evaluate the 
      possible involvement of visceral adiposity in the development of PCOS. METHODS: 
      Ten PCOS patients and nine control samples were included. We used two-dimensional 
      difference gel electrophoresis (2D-DIGE) followed by in-gel digestion, and mass 
      spectrometry (MS) of selected protein spots. RESULTS: The 2D-DIGE technology 
      allowed the analysis of approximately 1840 protein spots in the comparative study 
      of control and patient proteomes, revealing 15 statistically significant spot 
      changes (>2-fold, P < 0.05). Unambiguous protein identification was achieved for 
      9 of these 15 spots by MS. This preliminary study revealed differences in 
      expression of proteins that may be involved in lipid and glucose metabolism, 
      oxidative stress processes and adipocyte differentiation; they include 
      proapolipoprotein Apo-A1, annexin V, glutathione S-transferase M3 (GSTM3), 
      triosephosphate isomerase, peroxiredoxin 2 isoform a, actin and adipocyte plasma 
      membrane-associated protein. The most relevant finding was an increase of GSTM3 
      in the omental fat of PCOS patients confirming previous studies conducted by our 
      group. CONCLUSIONS: Proteomic analysis of omental fat reveals differential 
      expression of several proteins in PCOS patients and non-hyperandrogenic women 
      presenting with morbid obesity. The application of this novel methodology adds 
      further evidence to support the role of visceral adiposity in the pathogenesis of 
      PCOS.
FAU - Corton, Marta
AU  - Corton M
AD  - Instituto de Investigaciones Biomedicas, Consejo Superior de Investigaciones 
      Cientificas and Universidad Autonoma de Madrid, E-28029 Madrid, Spain.
FAU - Botella-Carretero, Jose I
AU  - Botella-Carretero JI
FAU - Lopez, Juan A
AU  - Lopez JA
FAU - Camafeita, Emilio
AU  - Camafeita E
FAU - San Millan, Jose L
AU  - San Millan JL
FAU - Escobar-Morreale, Hector F
AU  - Escobar-Morreale HF
FAU - Peral, Belen
AU  - Peral B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071222
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
RN  - 0 (Annexin A5)
RN  - EC 1.11.1.15 (PRDX2 protein, human)
RN  - EC 1.11.1.15 (Peroxiredoxins)
RN  - EC 2.5.1.18 (GSTM3 protein, human)
RN  - EC 2.5.1.18 (Glutathione Transferase)
SB  - IM
MH  - Abdominal Fat/*metabolism
MH  - Adult
MH  - Annexin A5/metabolism
MH  - Electrophoresis, Gel, Two-Dimensional/methods
MH  - Female
MH  - Gene Expression Profiling
MH  - Glutathione Transferase/metabolism
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Obesity, Morbid/physiopathology
MH  - Omentum/*metabolism
MH  - Oxidative Stress
MH  - Peroxiredoxins/metabolism
MH  - Polycystic Ovary Syndrome/etiology/*physiopathology
MH  - *Proteomics
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Tandem Mass Spectrometry
EDAT- 2007/12/25 09:00
MHDA- 2008/03/18 09:00
CRDT- 2007/12/25 09:00
PHST- 2007/12/25 09:00 [pubmed]
PHST- 2008/03/18 09:00 [medline]
PHST- 2007/12/25 09:00 [entrez]
AID - dem380 [pii]
AID - 10.1093/humrep/dem380 [doi]
PST - ppublish
SO  - Hum Reprod. 2008 Mar;23(3):651-61. doi: 10.1093/humrep/dem380. Epub 2007 Dec 22.