PMID- 18156744
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20120823
LR  - 20191110
IS  - 1598-6535 (Print)
IS  - 1598-6535 (Linking)
VI  - 26
IP  - 5
DP  - 2006 Oct
TI  - [Comparison of the rate of detection of immunoglobulin heavy chain gene 
      rearrangement by fluoresecence in situ hybridization probes in multiple 
      myeloma.].
PG  - 317-22
AB  - BACKGROUND: Immunoglobulin heavy chain (IgH) gene rearrangement, which is 
      frequently observed in multiple myeloma, can now be detected easily by using a 
      fluorescence in situ hybridization (FISH) method. The aim of this study was to 
      determine the detection rate and compare the utility of the three most commonly 
      used probes: IGH/CCND1 dual color, dual fusion probe; IGH/BCL2 dual color, dual 
      fusion probe; and IGH dual color break apart rearrangement probe; all from Vysis 
      Products (Downers Grove, IL, USA). METHODS: From October 1994 to July 2003, 99 
      patients were diagnosed as multiple myeloma at Seoul National University 
      Hospital, Asan Medical Center and Gachon University Gil hospital. We applied the 
      three different probes of IgH FISH on bone marrow specimens from the 99 Korean 
      patients with multiple myeloma to detect IgH gene rearrangement. RESULTS: 
      Forty-one (41.4%) of the 99 patients had IgH gene rearrangement. Of those 41 
      patients, 23 (56.1%) showed positive to all three probes, but the remaining 18 
      (43.9%) showed a discrepancy between the three probes: 13 (72.2%) of the 18 
      patients were only positive to the IGH dual color break apart rearrangement probe 
      and the detection rate was 39.6% on the average. CONCLUSIONS: These results 
      demonstrate that IGH dual color break apart rearrangement probe is superior to 
      the other two probes in qualitative and quantitative ways. Thus, we recommend IGH 
      dual color break apart rearrangement probe for the diagnosis and monitoring of 
      multiple myeloma.
FAU - Chung, Hye Yoon
AU  - Chung HY
AD  - Department of Laboratory Medicine, College of Medicine, Seoul National 
      University, Seoul, Korea. soonlee@plaza.snu.ac.kr.
FAU - See, Cha Ja
AU  - See CJ
FAU - Choi, Jung Eun
AU  - Choi JE
FAU - Min, Hyun Jung
AU  - Min HJ
FAU - Cho, Han Ik
AU  - Cho HI
FAU - Lee, Dong Soon
AU  - Lee DS
LA  - kor
PT  - English Abstract
PT  - Journal Article
PL  - Korea (South)
TA  - Korean J Lab Med
JT  - The Korean journal of laboratory medicine
JID - 101322822
EDAT- 2007/12/25 09:00
MHDA- 2007/12/25 09:01
CRDT- 2007/12/25 09:00
PHST- 2007/12/25 09:00 [pubmed]
PHST- 2007/12/25 09:01 [medline]
PHST- 2007/12/25 09:00 [entrez]
AID - 200610317 [pii]
AID - 10.3343/kjlm.2006.26.5.317 [doi]
PST - ppublish
SO  - Korean J Lab Med. 2006 Oct;26(5):317-22. doi: 10.3343/kjlm.2006.26.5.317.