PMID- 18160324
OWN - NLM
STAT- MEDLINE
DCOM- 20080429
LR  - 20211203
IS  - 1098-8823 (Print)
IS  - 1098-8823 (Linking)
VI  - 85
IP  - 1-2
DP  - 2008 Feb
TI  - PGF2alpha-associated vascular smooth muscle hypertrophy is ROS dependent and 
      involves the activation of mTOR, p70S6k, and PTEN.
PG  - 49-57
AB  - Prostaglandin F2alpha (PGF2alpha) increases reactive oxygen species (ROS) and 
      induces vascular smooth muscle cell (VSMC) hypertrophy by largely unknown 
      mechanism(s). To investigate the signaling events governing PGF2alpha-induced 
      VSMC hypertrophy we examined the ability of the PGF2alpha analog, fluprostenol to 
      elicit phosphorylation of Akt, the mammalian target of rapamycin (mTOR), 
      ribosomal protein S6 kinase (p70S6k), glycogen synthase kinase-3beta (GSK-3beta), 
      phosphatase and tensin homolog (PTEN), extracellular signal-regulated kinase 1/2 
      (ERK1/2) and Jun N-terminal kinase (JNK) in growth arrested A7r5 VSMC. 
      Fluprostenol-induced hypertrophy was associated with increased ROS, mTOR 
      translocation from the nucleus to the cytoplasm, along with Akt, mTOR, GSK-3beta, 
      PTEN and ERK1/2 but not JNK phosphorylation. Whereas inhibition of 
      phosphatidylinositol 3-kinase (PI3K) by LY-294002 blocked fluprostenol-induced 
      changes in total protein content, pre-treatment with rapamycin or with the MEK1/2 
      inhibitor U0126 did not. Taken together, these findings suggest that 
      fluprostenol-induced changes in A7r5 hypertrophy involve mTOR translocation and 
      occur through PI3K-dependent mechanisms.
FAU - Rice, K M
AU  - Rice KM
AD  - Department of Biological Sciences, Marshall University, Huntington, WV 
      25755-1090, USA. rice@marshall.edu
FAU - Uddemarri, S
AU  - Uddemarri S
FAU - Desai, D H
AU  - Desai DH
FAU - Morrison, R G
AU  - Morrison RG
FAU - Harris, R
AU  - Harris R
FAU - Wright, G L
AU  - Wright GL
FAU - Blough, E R
AU  - Blough ER
LA  - eng
GR  - R15 AG027103/AG/NIA NIH HHS/United States
GR  - P20 RR016477/RR/NCRR NIH HHS/United States
GR  - R15 AG027103-01/AG/NIA NIH HHS/United States
GR  - RR16477/RR/NCRR NIH HHS/United States
GR  - AG-20370/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20071117
PL  - United States
TA  - Prostaglandins Other Lipid Mediat
JT  - Prostaglandins & other lipid mediators
JID - 9808648
RN  - 0 (Phosphoinositide-3 Kinase Inhibitors)
RN  - 0 (Prostaglandins F, Synthetic)
RN  - 0 (Reactive Oxygen Species)
RN  - 358S7VUE5N (fluprostenol)
RN  - B7IN85G1HY (Dinoprost)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.1.1 (mTOR protein, mouse)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 70-kDa)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 3.1.3.67 (PTEN Phosphohydrolase)
RN  - EC 3.1.3.67 (Pten protein, mouse)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cell Line
MH  - Dinoprost/agonists/*physiology
MH  - Fluorescent Antibody Technique
MH  - MAP Kinase Signaling System
MH  - Microscopy, Fluorescence
MH  - Muscle, Smooth, Vascular/*pathology
MH  - PTEN Phosphohydrolase/*metabolism
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Phosphoinositide-3 Kinase Inhibitors
MH  - Prostaglandins F, Synthetic/pharmacology
MH  - Protein Kinases/drug effects/*metabolism
MH  - Rats
MH  - Reactive Oxygen Species/*metabolism
MH  - Ribosomal Protein S6 Kinases, 70-kDa/antagonists & inhibitors/*metabolism
MH  - TOR Serine-Threonine Kinases
PMC - PMC2277510
MID - NIHMS39450
EDAT- 2007/12/28 09:00
MHDA- 2008/04/30 09:00
PMCR- 2009/02/01
CRDT- 2007/12/28 09:00
PHST- 2006/05/08 00:00 [received]
PHST- 2007/07/12 00:00 [revised]
PHST- 2007/10/26 00:00 [accepted]
PHST- 2007/12/28 09:00 [pubmed]
PHST- 2008/04/30 09:00 [medline]
PHST- 2007/12/28 09:00 [entrez]
PHST- 2009/02/01 00:00 [pmc-release]
AID - S1098-8823(07)00122-0 [pii]
AID - 10.1016/j.prostaglandins.2007.10.009 [doi]
PST - ppublish
SO  - Prostaglandins Other Lipid Mediat. 2008 Feb;85(1-2):49-57. doi: 
      10.1016/j.prostaglandins.2007.10.009. Epub 2007 Nov 17.