PMID- 18160336
OWN - NLM
STAT- MEDLINE
DCOM- 20080618
LR  - 20220310
IS  - 1383-5769 (Print)
IS  - 1383-5769 (Linking)
VI  - 57
IP  - 2
DP  - 2008 Jun
TI  - A survey of malarial infection in endemic areas of Savannakhet province, Lao PDR 
      and comparative diagnostic efficiencies of Giemsa staining, acridine orange 
      staining, and semi-nested multiplex PCR.
PG  - 143-9
AB  - Malaria remains one of the most important parasitic diseases in Lao PDR, 
      especially in forested rural areas. Knowing the rate of infection using highly 
      sensitive and specific methods, and the factors related to malarial infection, 
      may be helpful in reducing the infection and mortality rates. We aimed to study 
      the malarial infection rate by comparing three detection methods, i.e., Giemsa 
      staining, acridine orange (AO) staining and semi-nested multiplex PCR. The study 
      also included some factors related to malarial infection in the endemic areas of 
      Savannakhet province, Lao PDR. The respective malarial infection rates by Giemsa 
      staining, AO staining and semi-nested multiplex PCR in Houy Jang vs. Keng Thong 
      villages were 13.1 vs. 20.8, 16.2 vs. 25.4 and 20.8 vs. 30.8%. The infection rate 
      among children not over 10 years of age was higher than infection rate among the 
      older ages (p=0.002, Z-test for two proportions). The higher infection rates by 
      semi-nested multiplex PCR over Giemsa and AO staining suggest the existence of 
      many subclinical cases with low level parasitemia, undetected by microscopic 
      techniques. We found no mixed infections using Giemsa or AO staining, but using 
      semi-nested multiplex PCR we found 1.2% (3/260) mixed P. falciparum and P. vivax 
      infections, suggesting that semi-nested multiplex PCR is suitable for detecting 
      malarial infection from endemic areas whose cases may have low parasitemia and/or 
      mixed infection. The factors significantly related to malarial infection from 260 
      questionnaires were: (1) children and young adults, (2) not having lived in the 
      area more than 5 years, and (3) not using a mosquito net over the bed, indicating 
      an increased risk of new residents of contracting malaria and a need to promote 
      bed nets.
FAU - Khaminsou, Naly
AU  - Khaminsou N
AD  - College of Health Technology, Vientiane, Lao Democratic People's Republic.
FAU - Kritpetcharat, Onanong
AU  - Kritpetcharat O
FAU - Daduang, Jureerut
AU  - Daduang J
FAU - Kritpetcharat, Panutas
AU  - Kritpetcharat P
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071124
PL  - Netherlands
TA  - Parasitol Int
JT  - Parasitology international
JID - 9708549
RN  - 0 (Azure Stains)
RN  - 0 (DNA, Protozoan)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Adolescent
MH  - Adult
MH  - Animals
MH  - Azure Stains
MH  - Child
MH  - DNA, Protozoan/analysis/isolation & purification
MH  - *Endemic Diseases
MH  - Female
MH  - Humans
MH  - Laos/epidemiology
MH  - Malaria/*diagnosis/*epidemiology/parasitology
MH  - Male
MH  - Middle Aged
MH  - Parasitology/methods
MH  - Plasmodium/classification/genetics/*isolation & purification
MH  - Polymerase Chain Reaction/*methods
MH  - Prevalence
MH  - Sensitivity and Specificity
MH  - Staining and Labeling/*methods
EDAT- 2007/12/28 09:00
MHDA- 2008/06/19 09:00
CRDT- 2007/12/28 09:00
PHST- 2007/06/19 00:00 [received]
PHST- 2007/11/09 00:00 [revised]
PHST- 2007/11/17 00:00 [accepted]
PHST- 2007/12/28 09:00 [pubmed]
PHST- 2008/06/19 09:00 [medline]
PHST- 2007/12/28 09:00 [entrez]
AID - S1383-5769(07)00153-5 [pii]
AID - 10.1016/j.parint.2007.11.003 [doi]
PST - ppublish
SO  - Parasitol Int. 2008 Jun;57(2):143-9. doi: 10.1016/j.parint.2007.11.003. Epub 2007 
      Nov 24.