PMID- 18160476
OWN - NLM
STAT- MEDLINE
DCOM- 20080318
LR  - 20220318
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Print)
IS  - 0019-9567 (Linking)
VI  - 76
IP  - 3
DP  - 2008 Mar
TI  - Differential uptake and processing of a Haemophilus influenzae P5-derived 
      immunogen by chinchilla dendritic cells.
PG  - 967-77
AB  - Dendritic cells (DCs) are potent antigen-presenting cells involved in the 
      initiation and modulation of immune responses after immunization via their 
      ability to process and present antigen to naive T cells. We wanted to examine the 
      role of DCs in the development of protective immunity against nontypeable 
      Haemophilus influenzae (NTHI)-induced experimental otitis media (OM) after 
      intranasal immunization of chinchillas with an NTHI P5-derived synthetic peptide 
      immunogen called LB1. As chinchilla DCs have not been described, we adapted 
      well-established protocols to induce the differentiation of chinchilla bone 
      marrow precursor cells into DCs, which resulted in cells that were 
      morphologically and phenotypically similar to DCs of other species. In vitro, 
      chinchilla DCs readily internalized LB1, upregulated expression of the maturation 
      markers CD80 and major histocompatibility complex class II, and presented 
      processed LB1 to primed CD3+ T cells, which resulted in antigen-specific T-cell 
      proliferation. In vivo, LB1-activated DCs trafficked from the chinchilla nasal 
      cavity primarily to the nasal-associated lymphoid tissues and were detected in 
      close proximity to CD3+ T cells within this lymphoid aggregate. These data are 
      the first to characterize chinchilla DCs and their functional properties. 
      Furthermore, they suggest an important role for chinchilla DCs in the development 
      of protective immunity against experimental NTHI-induced OM after intranasal 
      immunization.
FAU - Novotny, Laura A
AU  - Novotny LA
AD  - Center for Microbial Pathogenesis, The Research Institute at Nationwide 
      Children's Hospital, The Ohio State University College of Medicine, Columbus, OH 
      43205-2696, USA.
FAU - Partida-Sanchez, Santiago
AU  - Partida-Sanchez S
FAU - Munson, Robert S Jr
AU  - Munson RS Jr
FAU - Bakaletz, Lauren O
AU  - Bakaletz LO
LA  - eng
GR  - R01 DC003915/DC/NIDCD NIH HHS/United States
GR  - R01DC03915/DC/NIDCD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20071226
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Antigens, Bacterial)
RN  - 0 (B7-1 Antigen)
RN  - 0 (Bacterial Outer Membrane Proteins)
RN  - 0 (Haemophilus Vaccines)
RN  - 0 (Vaccines, Synthetic)
RN  - 149024-69-1 (OMPA outer membrane proteins)
SB  - IM
MH  - Animals
MH  - *Antigen Presentation
MH  - Antigens, Bacterial/immunology/*metabolism
MH  - B7-1 Antigen/genetics
MH  - Bacterial Outer Membrane Proteins/immunology/*metabolism
MH  - Cell Proliferation
MH  - Chemotaxis
MH  - Chinchilla
MH  - Dendritic Cells/chemistry/cytology/*immunology/metabolism
MH  - Gene Expression Regulation
MH  - Genes, MHC Class II
MH  - Haemophilus Vaccines/immunology
MH  - Haemophilus influenzae/*immunology
MH  - Otitis Media/immunology/prevention & control
MH  - T-Lymphocytes/immunology
MH  - Vaccines, Synthetic/immunology
PMC - PMC2258844
EDAT- 2007/12/28 09:00
MHDA- 2008/03/19 09:00
PMCR- 2008/07/01
CRDT- 2007/12/28 09:00
PHST- 2007/12/28 09:00 [pubmed]
PHST- 2008/03/19 09:00 [medline]
PHST- 2007/12/28 09:00 [entrez]
PHST- 2008/07/01 00:00 [pmc-release]
AID - IAI.01395-07 [pii]
AID - 1395-07 [pii]
AID - 10.1128/IAI.01395-07 [doi]
PST - ppublish
SO  - Infect Immun. 2008 Mar;76(3):967-77. doi: 10.1128/IAI.01395-07. Epub 2007 Dec 26.