PMID- 18160789
OWN - NLM
STAT- MEDLINE
DCOM- 20080118
LR  - 20071227
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 119
IP  - 1-2
DP  - 2007
TI  - Localization of single-copy sequences on chicken synaptonemal complex spreads 
      using fluorescence in situ hybridization (FISH).
PG  - 105-12
AB  - Synaptonemal complex (SC) spreads from bird oocytes and spermatocytes show the 
      complete chromosome complement and can be observed at the light microscope using 
      immunostaining of the proteins that compose the lateral elements. To investigate 
      the use of avian SC spreads as substrates for fluorescent in situ hybridization 
      (FISH) in combination with immunostaining, we applied two single-copy sequences 
      to chicken oocyte spreads. Signals for both target sequences were consistently 
      observed on the short arm of bivalent 1 in a large number of nuclei. Based on 
      previous data about the size of chromosome 1 and from measurements on probed SC 
      spreads, an estimate of the physical distance in Mb between each sequence and the 
      telomere was calculated. The crossover frequencies along SC 1 obtained by 
      immunolocalization of MLH1 foci during pachytene were used to calculate the 
      distances in cM to the target sequences and to compare this cytogenetic SC map 
      with the consensus linkage map for GGA1. The combination of SC-FISH and 
      immunostaining could be generally applied to obtain high-resolution mapping of 
      single-copy sequences in birds and, coupled with MLH1 crossover maps, it could be 
      a reliable approach to obtain genetic distances between markers to test the 
      genetic linkage maps generated from molecular markers.
CI  - Copyright (c) 2007 S. Karger AG, Basel.
FAU - Pigozzi, M I
AU  - Pigozzi MI
AD  - Instituto de Investigaciones en Reproduccion, Facultad de Medicina, Universidad 
      de Buenos Aires, Buenos Aires, Argentina. mpigozzi@fmed.uba.ar
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071214
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Nuclear Proteins)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/genetics
MH  - Animals
MH  - Base Sequence
MH  - Chickens/*genetics
MH  - Chromosomes/genetics
MH  - Gene Dosage/*genetics
MH  - *In Situ Hybridization, Fluorescence
MH  - Nuclear Proteins/genetics
MH  - Synaptonemal Complex/*genetics
EDAT- 2007/12/28 09:00
MHDA- 2008/01/19 09:00
CRDT- 2007/12/28 09:00
PHST- 2007/01/17 00:00 [received]
PHST- 2007/04/23 00:00 [accepted]
PHST- 2007/12/28 09:00 [pubmed]
PHST- 2008/01/19 09:00 [medline]
PHST- 2007/12/28 09:00 [entrez]
AID - 000109626 [pii]
AID - 10.1159/000109626 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2007;119(1-2):105-12. doi: 10.1159/000109626. Epub 2007 Dec 
      14.