PMID- 18160852
OWN - NLM
STAT- MEDLINE
DCOM- 20080506
LR  - 20071227
IS  - 1226-3613 (Print)
IS  - 1226-3613 (Linking)
VI  - 39
IP  - 6
DP  - 2007 Dec 31
TI  - Interleukin-10 endogenously expressed in microglia prevents 
      lipopolysaccharide-induced neurodegeneration in the rat cerebral cortex in vivo.
PG  - 812-9
AB  - A degree of brain inflammation is required for repair of damaged tissue, but 
      excessive inflammation causes neuronal cell death. Here, we observe that IL-10 is 
      expressed in LPS-injected rat cerebral cortex, contributing to neuronal survival. 
      Cells immunopositive for IL-10 were detected as early as 8 h post-injection and 
      persisted for up to 3 d, in parallel with the expression of IL-1beta, TNF-alpha, 
      and iNOS. Double immunofluorescence staining showed that IL-10 expression was 
      localized mainly in activated microglia. Next, we examined the neuroprotective 
      effects of IL-10 using IL-10 neutralizing antibody (IL-10NA). Blockade of IL-10 
      action caused a significant loss of neurons both 3 d and 7 d after LPS injection. 
      Further, the induction of mRNA species encoding IL-1beta, TNF-alpha, and iNOS, 
      reactive oxygen species (ROS) production, and NADPH oxidase activation, increased 
      after co-injection of LPS and IL-10NA, compared to the levels seen after 
      injection of LPS alone. Taken together, these results clearly suggest that 
      LPS-induced endogenous expression of IL-10 in microglia contributes to neuronal 
      survival by inhibiting brain inflammation.
FAU - Park, Keun Woo
AU  - Park KW
AD  - Neuroscience Graduate Program, Ajou University School of Medicine, Suwon 443-721, 
      Korea.
FAU - Lee, Hwan Goo
AU  - Lee HG
FAU - Jin, Byung Kwan
AU  - Jin BK
FAU - Lee, Yong Beom
AU  - Lee YB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Mol Med
JT  - Experimental & molecular medicine
JID - 9607880
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Reactive Oxygen Species)
RN  - 130068-27-8 (Interleukin-10)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
SB  - IM
MH  - Animals
MH  - Cerebral Cortex/drug effects/*pathology
MH  - Fluorescent Antibody Technique
MH  - Interleukin-10/immunology/*physiology
MH  - Lipopolysaccharides/*pharmacology
MH  - Microglia/cytology/*metabolism
MH  - Nerve Degeneration/pathology/*prevention & control
MH  - Neurons/cytology/drug effects/*metabolism
MH  - Nitric Oxide Synthase/genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reactive Oxygen Species/metabolism
EDAT- 2007/12/28 09:00
MHDA- 2008/05/07 09:00
CRDT- 2007/12/28 09:00
PHST- 2007/12/28 09:00 [pubmed]
PHST- 2008/05/07 09:00 [medline]
PHST- 2007/12/28 09:00 [entrez]
AID - 2007123115 [pii]
AID - 10.1038/emm.2007.88 [doi]
PST - ppublish
SO  - Exp Mol Med. 2007 Dec 31;39(6):812-9. doi: 10.1038/emm.2007.88.