PMID- 18161572
OWN - NLM
STAT- MEDLINE
DCOM- 20080407
LR  - 20131121
IS  - 0360-1234 (Print)
IS  - 0360-1234 (Linking)
VI  - 43
IP  - 1
DP  - 2008 Jan
TI  - Development of enzyme immunoassay for detection of DDT.
PG  - 44-9
AB  - Dichlorodiphenyltrichloroethane (DDT) is one of the persistent organic pollutants 
      (POPs) widely found in the environment and in the general population. In this 
      study, a direct competitive enzyme immunoassay (EIA) has been developed for the 
      quantitative analysis of DDT. To generate a specific polyclonal antibody for EIA, 
      p, p'-DDT was conjugated to porcine thyroglobulin for rabbit immunization. At 
      optimized EIA conditions, the standard curves ranged from 0.137 to 100 ng/mL with 
      the quantification limit of 0.41 ng/mL. The coefficients of variation (CV%) were 
      5.42-10.53% for intra-assay and 6.04-7.26% for inter-assay. Cross-reactivities 
      with DDT metabolites (DDTs, including o, p'-DDT, p, p'-DDD, o, p'-DDD, p, p'-DDE, 
      o, p'-DDE, p, p'-dichlorobenzophenone (DCBP), o, p'-DCBP) were investigated. The 
      polyclonal antibody showed relatively low and/or no cross-reactivity with these 
      compounds, and the assay was seen to be highly selective for p, p'-DDT. Moreover, 
      the DDTs could be ranked by their reactivity: DDT > DDD > DDE > DCBP. In 
      addition, the characterization of the polyclonal antibody indicated that the 
      antiserum possesses a high specificity for p, p'-isomers. The results indicated 
      that the developed EIA using this antibody could be a convenient and supplemental 
      analytical tool for monitoring DDT.
FAU - Hirano, Masashi
AU  - Hirano M
AD  - Faculty of Environmental and Symbiotic Sciences, Prefectural University of 
      Kumamoto, 3-1-100 Tsukide, Kumamoto, Japan.
FAU - Kitamura, Kazuyuki
AU  - Kitamura K
FAU - Kato, Ikuo
AU  - Kato I
FAU - Yanaihara, Chizuko
AU  - Yanaihara C
FAU - Iwamoto, Ken-ichi
AU  - Iwamoto K
FAU - Sekiyama, Makiko
AU  - Sekiyama M
FAU - Watanabe, Chiho
AU  - Watanabe C
FAU - Nakamoto, Takashi
AU  - Nakamoto T
FAU - Miyamoto, Nobukazu
AU  - Miyamoto N
FAU - Onishi, Yuta
AU  - Onishi Y
FAU - Arizono, Koji
AU  - Arizono K
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Environ Sci Health B
JT  - Journal of environmental science and health. Part. B, Pesticides, food 
      contaminants, and agricultural wastes
JID - 7607167
RN  - 0 (Antibodies)
RN  - 0 (Pesticide Residues)
RN  - 0 (Pesticides)
RN  - 0 (Soil Pollutants)
RN  - CIW5S16655 (DDT)
SB  - IM
MH  - Animals
MH  - Antibodies/immunology
MH  - Antibody Specificity
MH  - Carps/metabolism
MH  - Cross Reactions
MH  - DDT/*analysis
MH  - Environmental Monitoring/*methods
MH  - Environmental Pollution/*analysis
MH  - *Immunoenzyme Techniques/methods/standards
MH  - Isomerism
MH  - Pesticide Residues/*analysis
MH  - Pesticides/*analysis
MH  - Rabbits
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Soil Pollutants/analysis
EDAT- 2007/12/29 09:00
MHDA- 2008/04/09 09:00
CRDT- 2007/12/29 09:00
PHST- 2007/12/29 09:00 [pubmed]
PHST- 2008/04/09 09:00 [medline]
PHST- 2007/12/29 09:00 [entrez]
AID - 789007048 [pii]
AID - 10.1080/03601230701734964 [doi]
PST - ppublish
SO  - J Environ Sci Health B. 2008 Jan;43(1):44-9. doi: 10.1080/03601230701734964.