PMID- 18162069
OWN - NLM
STAT- MEDLINE
DCOM- 20080326
LR  - 20131121
IS  - 1530-0277 (Electronic)
IS  - 0145-6008 (Linking)
VI  - 32
IP  - 2
DP  - 2008 Feb
TI  - Dose-dependent deficits in dual interstimulus interval classical eyeblink 
      conditioning tasks following neonatal binge alcohol exposure in rats.
PG  - 277-93
AB  - BACKGROUND: Neonatal alcohol consumption in rats is widely used to model 
      cerebellar injury arising from 3rd-trimester human fetal alcohol exposure. Binge 
      alcohol exposure of 5 g/kg/day or more over postnatal days (PD) 4 to 9 in rats 
      damages the cerebellum and consequently impairs classical eyeblink conditioning 
      (EBC). The present study sought to identify deficits in EBC using doses lower 
      than those that have been reported previously following alcohol exposure limited 
      to PD4-9. Complex conditioned response (CR) timing tasks utilizing 2 
      interstimulus intervals (ISIs) were used to test the hypothesis that 3 g/kg/day 
      of alcohol would produce early onset and early peaked CRs, whereas 4 and 5 
      g/kg/day would impair CR acquisition. METHODS: Five neonatal treatment groups 
      were used: (1) undisturbed controls, (2) sham intubation controls, (3) 3 g/kg/day 
      of alcohol, (4) 4 g/kg/day of alcohol, or (5) 5 g/kg/day of alcohol. Intubations 
      occurred over PD4-9. In adulthood, rats were trained using ISI discrimination 
      (Experiment 1) or temporal uncertainty (Experiment 2) EBC tasks. In ISI 
      discrimination, 2 distinct conditioned stimuli (CSs; tone and light) are 
      reinforced with a periocular shock unconditioned stimulus (US) at 2 different 
      CS-US intervals. Temporal uncertainty is identical in design with the exception 
      that the same CS is presented at both CS-US intervals. RESULTS: Alcohol-exposed 
      subjects were impaired in CR acquisition in a task- and dose-dependent fashion. 
      CR deficits were most salient in the peak amplitude measure and occurred in both 
      tasks following alcohol exposure at 4 and 5 g/kg/day. Alcohol at a dosage of 3 
      g/kg/day impaired CR acquisition only in ISI discrimination. All alcohol doses 
      failed to produce short latency CRs in either task. Alcohol-exposed subjects 
      displayed later-onset and later-peaked CRs to the long-ISI CS in ISI 
      discrimination relative to controls. CONCLUSIONS: ISI discrimination training may 
      be ideal to identify CR deficits resulting from neonatal exposure to moderate 
      alcohol doses. Applications of this EBC task to humans may enable reliable early 
      identification and diagnosis of individuals with fetal alcohol spectrum 
      disorders.
FAU - Brown, Kevin L
AU  - Brown KL
AD  - Department of Psychology, University of Delaware, Newark, Delaware, USA. 
      kbrown@psych.udel.edu
FAU - Calizo, Lyngine H
AU  - Calizo LH
FAU - Stanton, Mark E
AU  - Stanton ME
LA  - eng
GR  - 1-F31-AA16250-01/AA/NIAAA NIH HHS/United States
GR  - 1-R01-AA11945/AA/NIAAA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20071221
PL  - England
TA  - Alcohol Clin Exp Res
JT  - Alcoholism, clinical and experimental research
JID - 7707242
RN  - 3K9958V90M (Ethanol)
SB  - IM
MH  - Alcoholic Intoxication/*physiopathology
MH  - Animals
MH  - Animals, Newborn
MH  - Cerebellum/*physiopathology
MH  - Conditioning, Classical/*physiology
MH  - Conditioning, Eyelid/*physiology
MH  - Discrimination Learning/physiology
MH  - *Disease Models, Animal
MH  - Dose-Response Relationship, Drug
MH  - Electromyography
MH  - Ethanol/blood
MH  - Female
MH  - Fetal Alcohol Spectrum Disorders/*physiopathology
MH  - Humans
MH  - Infant, Newborn
MH  - Male
MH  - Pregnancy
MH  - Rats
MH  - Rats, Long-Evans
MH  - Time Perception/*physiology
EDAT- 2007/12/29 09:00
MHDA- 2008/03/28 09:00
CRDT- 2007/12/29 09:00
PHST- 2007/12/29 09:00 [pubmed]
PHST- 2008/03/28 09:00 [medline]
PHST- 2007/12/29 09:00 [entrez]
AID - ACER579 [pii]
AID - 10.1111/j.1530-0277.2007.00579.x [doi]
PST - ppublish
SO  - Alcohol Clin Exp Res. 2008 Feb;32(2):277-93. doi: 
      10.1111/j.1530-0277.2007.00579.x. Epub 2007 Dec 21.