PMID- 18163473
OWN - NLM
STAT- MEDLINE
DCOM- 20080522
LR  - 20080402
IS  - 1552-4930 (Electronic)
IS  - 1552-4922 (Linking)
VI  - 73
IP  - 4
DP  - 2008 Apr
TI  - Losses of 3p14 and 9p21 as shown by fluorescence in situ hybridization are early 
      events in tumorigenesis of oral squamous cell carcinoma and already occur in 
      simple keratosis.
PG  - 305-11
LID - 10.1002/cyto.a.20504 [doi]
AB  - Tumorigenesis of oral squamous cell carcinoma (OSCC) has been postulated to 
      represent a multistep process driven by the accumulation of carcinogen-induced 
      genetic changes. Alterations of the 3p14 fragile site containing the fragile 
      histidine triade gene and of the 9p21 tumor suppressor locus containing 
      methylthioadenosine phosphorylase, p16 and p15 characteristically occur in oral 
      leukoplakia, a known precursor of OSCC, and are at present considered to indicate 
      the transition from simple keratosis (hyperplasia) to dysplasia. The aim of the 
      study was to evaluate the occurrence of losses of 3p14 and 9p21 and to evaluate 
      polysomies 3 and 9 in leukoplakias using highly sensitive fluorescence in situ 
      hybridization (FISH) probes. Examining 67 leukoplakias (24 hyperplasias, 33 
      dysplasias, 10 in situ carcinomas), control tissues of oral mucosa from infants 
      and adults as well as invasive carcinomas and normal epithelia of tumor patients 
      with locus specific FISH probes targeting 3p14 and 9p21, and centromeric probes 
      for chromosomes 3 and 9 we could demonstrate that losses of these sites appeared 
      very early in the tumorigenesis of OSCC and were already present in the great 
      majority of simple keratoses. Polysomy 3 occurring more frequently than polysomy 
      9 was characteristic of dysplasia and in situ carcinomas and thus seems to follow 
      losses of 3p14 and 9p21 during oral squamous cell carcinogenesis.
CI  - (c) 2007 International Society for Analytical Cytology.
FAU - Schwarz, Stephan
AU  - Schwarz S
AD  - Department of Pathology, University of Erlangen-Nuremberg, Erlangen, Germany. 
      stephan.schwarz@uk-erlangen.de
FAU - Bier, Johannes
AU  - Bier J
FAU - Driemel, Oliver
AU  - Driemel O
FAU - Reichert, Torsten E
AU  - Reichert TE
FAU - Hauke, Sven
AU  - Hauke S
FAU - Hartmann, Arndt
AU  - Hartmann A
FAU - Brockhoff, Gero
AU  - Brockhoff G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cytometry A
JT  - Cytometry. Part A : the journal of the International Society for Analytical 
      Cytology
JID - 101235694
SB  - IM
MH  - Adult
MH  - Carcinoma, Squamous Cell/*diagnosis/*genetics
MH  - Cell Transformation, Neoplastic
MH  - *Chromosomes, Human, Pair 3
MH  - *Chromosomes, Human, Pair 9
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Infant
MH  - Keratosis/*diagnosis/*genetics
MH  - Leukoplakia/genetics
MH  - Models, Biological
MH  - Mouth Mucosa/metabolism
MH  - Mouth Neoplasms/*diagnosis/*genetics
EDAT- 2008/01/01 09:00
MHDA- 2008/05/23 09:00
CRDT- 2008/01/01 09:00
PHST- 2008/01/01 09:00 [pubmed]
PHST- 2008/05/23 09:00 [medline]
PHST- 2008/01/01 09:00 [entrez]
AID - 10.1002/cyto.a.20504 [doi]
PST - ppublish
SO  - Cytometry A. 2008 Apr;73(4):305-11. doi: 10.1002/cyto.a.20504.